2026-10-12T17:36:47Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33903759 2026-09-17T17:27:41Z category_4 category_12 category_13 category_14 category_16 category_24 category_39 category_502 category_61 category_69 portal_5 item_type_3 month_year_09_2026

<p>Cell count per cluster and sample.</p> Maria Paula Zappia (17011257) Anton Westacott (25037565) Hannah Cooke (19183519) Rhianna Geary (25037568) Libby Travers (25037571) Lucia de Castro (25037574) Oliver Carty (25037577) Maxim V. Frolov (7943813) Biochemistry Cell Biology Genetics Molecular Biology Physiology Evolutionary Biology Ecology Astronomical and Space Sciences not elsewhere classified Developmental Biology Inorganic Chemistry sole phosphagen kinase single cell rna significantly affected compared low atp level div >< p cell cycle progression maintaining atp homeostasis indirect flight muscle supports energy homeostasis seq cell clusters proper sarcomere maturation flight muscle development depleting argk1 specifically argk1 knockdown causes sarcomere maturation energy homeostasis muscle development energy turnover muscle growth wing disc variable rates support myofibrillogenesis severely disrupted overall composition nadh ratio myoblasts differentiation muscles lead mitochondrial morphogenesis marker expression less understood least one isoforms localizes essential role energetic demand early states drosophila </ developing myofibers depleted myoblasts data reveal associated myoblasts animal lethality also necessary <div><p>In <i>Drosophila</i>, Arginine kinase 1 (Argk1) is involved in maintaining ATP homeostasis during bursts of activity in tissues with high and variable rates of energy turnover such as muscle. However, its role beyond stress conditions is less understood. Argk1 is the sole phosphagen kinase with dynamic expression throughout flight muscle development. Here, we show that at least one of the <i>Argk1</i> isoforms localizes to mitochondria in the developing myofibers, and its function is also necessary for proper flight muscle development. Depleting Argk1 specifically in the muscles lead to low ATP level and NAD + /NADH ratio, indicative of defects in energy homeostasis, and results in animal lethality. In the wing disc-associated myoblasts, Argk1 knockdown causes a reduction in cell size without changes in cell cycle progression. Single cell RNA-sequencing (scRNA-seq) revealed that the transcriptomes of undifferentiated and differentiating Argk1-depleted myoblasts are not significantly affected compared to control. Furthermore, based on the marker expression and overall composition of scRNA-seq cell clusters, the early states of myoblasts differentiation are not severely disrupted in Argk1-depleted myoblasts. Nonetheless, Argk1 knockdown severely impacts later stages of muscle development. Remarkably, Argk1-depleted muscles completely lack spontaneous muscle contractions, which are required for proper sarcomere maturation in the formation of the indirect flight muscle. Accordingly, Argk1-depleted muscles showed defects related to sarcomere maturation, and mitochondrial morphogenesis; thus, leading to a severe reduction in muscle growth. Therefore, our data reveal an essential role for Argk1 in flight muscle development, presumably by sustaining local ATP levels to meet the energetic demand to support myofibrillogenesis, muscle growth and proper flight muscle function.</p></div> 2026-09-17T17:40:03Z Dataset Dataset 10.1371/journal.pgen.1012304.s008 https://figshare.com/articles/dataset/_p_Cell_count_per_cluster_and_sample_p_/33903759 CC BY 4.0