2026-10-13T01:55:20Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33903540 2026-09-17T17:25:43Z category_1 category_12 category_13 category_15 category_16 category_19 category_39 category_46 category_734 portal_5 item_type_3 month_year_09_2026

<p>Raw data.</p> Yuan Ding (617135) Xiang Wang (127867) Shihua Zong (25037415) Qiong Wen (8087294) Luyang Zhang (5677079) Biophysics Cell Biology Genetics Neuroscience Physiology Pharmacology Ecology Immunology Biological Sciences not elsewhere classified normalizing dynamic balance mechanistic framework linking work highlights iq related chondrocyte impairment div >< p modifying therapies targeting iq binds chondrocyte anabolic stabilizing activities promising candidate overexpression abolishes oa ), mitigates pro key mediator high affinity coupling anti catabolic imbalance aberrant inflammation <div><p>Osteoarthritis (OA), a prevalent degenerative joint disorder, is driven by chondrocyte anabolic-catabolic imbalance, aberrant inflammation, and mitochondrial dysfunction. Disease-modifying therapies targeting these pathological processes remain scarce, limiting effective clinical intervention. Herein, we demonstrate an isoquercitrin (IQ)-based chondroprotective strategy that rectifies osteoarthritis-related chondrocyte impairment by targeting <i>AKT1</i>. IQ first reverses IL-1β-induced chondrocyte proliferation arrest, shifts metabolic homeostasis toward anabolism, and mitigates pro-inflammatory responses. It simultaneously restores mitochondrial homeostasis by normalizing dynamic balance. Mechanistically, IQ binds to AKT1 with high affinity, and <i>AKT1</i> overexpression abolishes its protective effects—confirming <i>AKT1</i> as a key mediator. This approach re-establishes chondrocyte functional homeostasis by coupling anti-inflammatory, metabolic-regulatory, and mitochondrial-stabilizing activities. This work highlights IQ as a promising candidate for osteoarthritis intervention and provides a mechanistic framework linking <i>AKT1</i> targeting to chondroprotection.</p></div> 2026-09-17T17:25:25Z Dataset Dataset 10.1371/journal.pone.0357989.s014 https://figshare.com/articles/dataset/_p_Raw_data_p_/33903540 CC BY 4.0