2026-10-09T19:26:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33895450 2026-09-18T12:56:45Z category_24244 category_24271 item_type_3 month_year_09_2026

Supplementary datasets for <b>Resolving the identities of salmonid </b><b><i>Sphaerospora </i></b><b>and </b><b><i>Myxidium</i></b><b> infections using combined morphological and molecular evidence</b> Pavla Sojkova (25021036) Pavla Bartosova-Sojkova (5024348) Pavla Bartošová-Sojková (544270) Animal systematics and taxonomy Phylogeny and comparative analysis Myxozoa mixed infections SSU rDNA phylogeny renal myxosporidiosis integrative taxonomy erroneous sequence assignments Sphaerospora Myxidium Fish <p dir="ltr">Salmonid fishes harbour diverse urinary and biliary myxozoans, but the identities of several historically reported <i>Sphaerospora</i> and <i>Myxidium</i> infections remain uncertain because morphology, tissue localization, and molecular data have not always been provided in a cohesive diagnosis. We re-examined selected salmonid myxozoan infections using combined morphological and SSU rDNA sequence data, focusing on incongruous sequence evidence assigned to <i>Sphaerospora oncorhynchi</i> and <i>Sphaerospora elwhaiensis</i>. We generated new sequences of both species, which clustered within <i>Sphaerospora</i> sensu stricto with other renal sphaerosporids from salmonids, confirming their generic placement. In contrast, the previously published <i>S. oncorhynchi</i> sequence grouped with <i>Myxidium</i> isolates in the <i>Myxidium lieberkuehni</i> (<i>Myxidium</i> sensu stricto) clade, whereas the published <i>S. elwhaiensis</i> sequence clustered within the hepatic biliary clade, indicating that both records likely originated from co-infecting myxidiids. Additional sequencing of salmonid kidney-derived myxidiids revealed two morphologically and phylogenetically distinct lineages within the <i>Myxidium</i> sensu stricto clade. A shorter, stouter morphotype was consistent with <i>M. minteri</i>-like infections, whereas a more elongate morphotype with pointed spore ends corresponded to <i>M. salvelini</i>-like infections. The latter lineage included both the previously published sequence assigned to <i>S. oncorhynchi</i> and the CKX parasite associated with proliferative renal myxosporidiosis in coho salmon, supporting the interpretation of CKX as a likely proliferative stage of an <i>M. salvelini</i>-like parasite. Gallbladder infections from coho salmon were identified as <i>Myxidium truttae</i>. These results demonstrate that mixed infections can cause erroneous sequence assignments and that phylogenetic context is important to consider, particularly if in conflict with well-supported clades.</p><p><br></p> 2026-09-18T12:56:45Z Dataset Dataset 10.6084/m9.figshare.33895450.v1 https://figshare.com/articles/dataset/Supplementary_datasets_for_b_Resolving_the_identities_of_salmonid_b_b_i_Sphaerospora_i_b_b_and_b_b_i_Myxidium_i_b_b_infections_using_combined_morphological_and_molecular_evidence_b_/33895450 CC BY 4.0 Open Access after 2027-09-30