2026-10-14T12:39:52Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33875776 2026-09-17T08:04:22Z portal_1197 item_type_3 month_year_09_2026

Data to support "Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation" Yanan Gao (360685) Akalabya Bissoyi (13256682) Nina L. H. Kinney (7828472) Thomas F. Whale (1362690) Qiongyu Guo (757990) Matthew I. Gibson (1273788) Proline Nucleation Ice Cell aggregation Cryopreservation of organs, tissues, etc. Migrated from ePrints Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.<br><br>Dataset file to support the article published in Chemical Communications 2023-08-24T00:00:00Z Dataset Dataset 10.82444/warw.33875776.v1 https://figshare.com/articles/dataset/Data_to_support_Proline-conditioning_and_chemically-programmed_ice_nucleation_protects_spheroids_during_cryopreservation_/33875776 CC BY 4.0