2026-10-14T11:40:14Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33871870 2026-09-17T05:33:12Z category_696 portal_316 item_type_3 month_year_09_2026

Table 1_Neutrophil extracellular traps as a potential therapeutic target in inflammatory bowel disease: a systematic review and meta-analysis.docx Yi-Jun Wu (471763) Yao Huang (216100) Hao Fang (69476) Bing-Yan Gu (25002049) Hong-Cheng Ge (25002052) Ru-Yi Xu (25002055) Jia-Min Qian (25002058) Dan-Ping Qin (25002061) Genetic Immunology inflammatory bowel disease meta-analysis NETs modulation neutrophil extracellular traps preclinical model targeted therapy Background<p>Elevated Neutrophil extracellular traps (NETs) associated biomarkers have been documented in inflammatory bowel disease (IBD) patients and preclinical models, but the therapeutic potential of targeting NETs remains unclear. This systematic review summarizes NETs-associated biomarkers in IBD patients and evaluates NETs-targeted interventions in preclinical models.</p>Methods<p>Following PRISMA guidelines, we searched databases up to November 2025. Clinical and preclinical studies assessing NETs expression and targeted modulation in IBD were included. Standardized mean difference (SMD) and 95% confidence interval (CI) were calculated using random-effects models.</p>Results<p>Forty-eight studies (12 clinical, 31 pre-clinical, 5 mixed) were identified. In clinical studies, NETs-associated biomarkers were elevated in the colonic mucosa and blood compared with healthy controls, and higher NETs burden was associated with more severe disease severity and poorer prognosis in IBD patients. In preclinical studies, direct NETs inhibition and degradation reduced disease activity index (SMD = -1.00 and -1.71, P< 0.05) and histological scores (SMD = -1.70 and -2.95, P< 0.01), and reversed colon shortening (P< 0.05). NETs modulation restored epithelial barrier by increasing occludin expression (SMD = 1.35 and 2.59, P< 0.01) and reducing intestinal permeability (P<0.05). Interventions suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ; P< 0.05) and increased anti-inflammatory cytokines (TGF-β, IL-10; P< 0.05). Subgroup analyses suggested administration route, modeling methods, and sample types may contribute to heterogeneity, although testing for subgroup differences was limited by the small number of studies. Sensitivity analyses indicated that pooled estimates for most outcomes were relatively stable, although several outcomes were not robust in sensitivity analyses after excluding individual studies. These findings should therefore be interpreted with caution.</p>Conclusion<p>NETs accumulation is a hallmark pathological feature of IBD. In preclinical IBD models, targeted NETs modulation ameliorates colitis by restoring the intestinal barrier integrity and suppressing inflammatory cascades. Further high-quality preclinical and translational studies are needed to define standardized detection methods, optimal dosing, and delivery strategies for clinical translation.</p>Systematic review registration<p>https://www.crd.york.ac.uk/PROSPERO, identifier CRD420261365119.</p> 2026-09-17T05:33:12Z Dataset Dataset 10.3389/fimmu.2026.1935261.s001 https://figshare.com/articles/dataset/Table_1_Neutrophil_extracellular_traps_as_a_potential_therapeutic_target_in_inflammatory_bowel_disease_a_systematic_review_and_meta-analysis_docx/33871870 CC BY 4.0