2026-10-11T18:23:39Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33870456 2026-09-17T04:06:03Z category_1 category_4 category_12 category_13 category_14 category_21 category_873 category_46 category_734 category_64 portal_63 item_type_3 month_year_09_2026

Toxicological Exploration of Collagenase and Benzonase on Rat Precision-Cut Liver Slices Janine Stam (13838685) Peter Olinga (584919) Aswin Menke (10323020) Pieter Annaert (5799536) Klaas Nico Faber (7821194) Peter L. Horvatovich (600604) Rainer Bischoff (600606) Justina C. Wolters (11211850) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Biotechnology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Cancer sized particles involved observed upon addition ex vivo </ cut liver slices ben ), along liver model evs ), ben ), toxicological exploration study emphasizes rat precision proteins related protein synthesis protein content intercellular communication extracellular vesicles extracellular matrix extracellular dna ev recovery ev isolation enzymes separately enzymatic treatments energy production decreased ribosomal cell viability ben decreased ben ). allows isolation Precision-cut liver slices (PCLSs) are an <i>ex vivo</i> liver model that allows isolation of extracellular vesicles (EVs), which are nano-sized particles involved in intercellular communication. However, EV recovery from PCLSs is hindered by adhesion to the extracellular matrix and macromolecules such as extracellular DNA. Enzymatic treatments, such as collagenase (COL) and the endonuclease benzonase (BEN), can aid their release, but potential effects on the tissue are unclear. Here, the effect of these enzymes separately, as well as combined, was analyzed to determine their effects on morphology, protein content, and the proteome of the PCLS. The enzymes showed minimal effects on cell viability and protein content after two different incubation times, whereas morphological analysis confirmed collagen degradation by COL and COL(BEN), along with decreased ribosomal and secretory secretory protein levels. BEN decreased the levels of proteins related to protein synthesis and degradation. Increased levels of proteins related to detoxification and energy production were observed upon addition of COL, BEN, and COL(BEN). This study emphasizes the potential and risks for the use of collagenase and benzonase for EV isolation from the PCLS. 2026-09-17T00:00:00Z Dataset Dataset 10.1021/acs.jproteome.5c01232.s002 https://figshare.com/articles/dataset/Toxicological_Exploration_of_Collagenase_and_Benzonase_on_Rat_Precision-Cut_Liver_Slices/33870456 CC BY-NC 4.0