2026-10-12T19:58:51Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/33867803
2026-09-16T18:00:20Z
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<p>Primers used for RT-qPCR.</p>
Chenyu Li (120599)
Yuxi Zhao (1797436)
Yuchen Zhang (524816)
Shuai Li (65944)
Longfei Chen (2159455)
Xiangfei Xu (5637917)
Tong Ding (1884523)
Yuanqing Wang (651429)
Xiang Gao (4077)
Zhaoyu Lin (170763)
Guanning Su (24998906)
Ankang Hu (12073505)
Liurong Fang (70706)
Qi Su (168460)
Quangang Chen (280646)
Yanrong Zhou (4841061)
Shaobo Xiao (38110)
Biochemistry
Microbiology
Cell Biology
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Mental Health
Infectious Diseases
Virology
immunomodulatory protein activated
gsdma_1 – 247
restricts viral replication
mortality upon infection
div >< p
mediated dual cleavage
viral load
triggers pyroptosis
tissue damage
terminal fragment
specific role
potential target
novel mechanism
inducing pyroptosis
findings suggest
findings reveal
disease severity
although gasdermin
active n
<div><p>Although Gasdermin A (GSDMA) drives inflammation by inducing pyroptosis, its specific role in antiviral defense remains unclear. Here we identify GSDMA as an immunomodulatory protein activated in response to coronavirus (CoV) infection. Specifically, CoV-encoded protease nsp5 cleaves GSDMA at two conserved glutamine sites, Q247 and Q187. Cleavage at Q247 liberates an active N-terminal fragment (GSDMA_1–247) that triggers pyroptosis, promotes inflammation, and restricts viral replication. In contrast, cleavage at the alternative site Q187 attenuates this function. Using <i>Gsdma</i><sup>-/-</sup> mice, we show that GSDMA deficiency increases viral loads but reduces inflammation, tissue damage, and mortality upon infection. These findings suggest that disease severity is driven more by inflammation than by viral load. Our findings reveal a novel mechanism of antiviral immunity and inflammatory regulation via CoV nsp5-mediated dual cleavage of GSDMA, highlighting a potential target for combined antiviral and anti-inflammatory therapies.</p></div>
2026-09-16T17:53:51Z
Dataset
Dataset
10.1371/journal.ppat.1014602.s008
https://figshare.com/articles/dataset/_p_Primers_used_for_RT-qPCR_p_/33867803
CC BY 4.0