2026-10-14T14:10:09Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33867590 2026-09-16T17:53:28Z category_1 category_8 category_12 category_21 category_873 category_46 category_64 category_132 category_134 portal_5 item_type_2 month_year_09_2026

<p>Dynamic association of Dr⁺ <i>E. coli</i> with calcium oxalate crystals in artificial urine.</p> Michalina Nagórka (24998945) Beata Zalewska-Piątek (738992) Adam Iwanicki (553559) Paweł Wityk (527516) Slawomir Jakiela (5180570) Weronika Switlik (24998948) Piotr Bruździak (8275656) Miłosz Wieczór (527514) Vanessa Liévin-Le Moal (24998951) Joanna Raczak-Gutknecht (8418300) Przemysław Gnatowski (24998954) Mateusz Ficek (3909271) Angelika Łepek (24998957) Magdalena Narajczyk (710511) Rafał Piątek (738993) Biophysics Microbiology Cell Biology Biotechnology Chemical Sciences not elsewhere classified Immunology Cancer Infectious Diseases Virology variable fimbrial expression simplified laboratory model preserved despite phase particularly hydrophilic glass mechanistic analyses performed markedly alters whole indicating steric exclusion fimbrial mesh functioned escherichia coli </ div >< p combining quantitative proteomics although traditionally described specialized surface appendages principal mineral component mediates caox recognition based adhesion assays bacterial surface architecture selective molecular filter including dr fimbriae surface physicochemistry principal physicochemical caox ), beyond adhesion bacterial cell dr fimbriae whereas infection urinary tract urinary conditions upec within term persistence strongly associated strains rely smaller phages small solutes remained unclear recurrent infection positive subpopulation physicochemical behavior largely abolished large bacteriophages kidney stones isolated filaments functional characterization findings establish computational modeling clinical isolate cell properties calcium oxalate broader contribution <p>Representative time-lapse phase-contrast recordings showing interactions of Dr⁺ <i>E. coli</i> AAEC191A with CaOx monohydrate crystals in AU (pH 5.7). Overnight cultures were diluted in AU to OD₆₀₀ = 0.025, supplemented with CaOx to a final concentration of 0.05% (w/v), and incubated under static conditions at 37 °C. The video shows four independent biological experiments recorded after 2 h of incubation. Images were acquired for 10 s at 0.1 s intervals using phase-contrast microscopy. Bacteria display characteristic polar attachment to CaOx crystals, forming radial arrangements around crystal surfaces. Collective bacterial motility results in visible oscillations and occasional displacement of crystals along the bottom surface. Recordings were acquired using an Olympus IX73 inverted microscope equipped with a UPlanFL N 20×/0.30 objective and a Hamamatsu Photonics ORCA-Flash2.8 camera.</p> <p>(MP4)</p> 2026-09-16T17:55:57Z Dataset Media 10.1371/journal.ppat.1014588.s025 https://figshare.com/articles/media/_p_Dynamic_association_of_Dr_sup_sup_i_E_coli_i_with_calcium_oxalate_crystals_in_artificial_urine_p_/33867590 CC BY 4.0