2026-10-14T04:18:48Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33866376 2026-09-16T17:38:22Z category_4 category_12 category_13 category_14 category_16 category_46 category_61 category_64 category_122 portal_5 item_type_3 month_year_09_2026

<p>List of differentially expressed genes (DEGs).</p> Sandhya Yadav (7699022) Tapan Sharma (16506867) Monserrat Olea-Flores (9012851) Teresita Padilla-Benavides (2632399) Anthony N. Imbalzano (284952) Biochemistry Cell Biology Genetics Molecular Biology Physiology Immunology Developmental Biology Cancer Mental Health differentiating c2c12 myoblasts containing mammalian swi >- deficient cells driven chromatin remodeling dependent cues via regulated pathways consistent homologue brm atpase direct downstream target defining transcription factors cycle gene programs essential differentiation determinant dependent chromatin remodeling impaired myoblast differentiation disrupted myoblast differentiation div >< p cn signaling converge findings identify tceal7 myoblast differentiation regulated target dependent kinase chromatin modifiers transcription factor identify tceal7 cycle control calcineurin signaling cn suppressed cn ); transcriptional control tceal7 protein tceal7 </ significantly reduced responsive regulators promoter increased phosphatase calcineurin pharmacological inhibition nuclear accumulation necessary component myogenin </ integrating calcium including changes data suggest coordinated activity <div><p>The transcriptional control of skeletal muscle differentiation requires the coordinated activity of lineage-defining transcription factors, signal-responsive regulators, chromatin modifiers, and ATP-dependent chromatin remodeling enzymes. Here, we identify TCEAL7, a member of the X-linked, poorly characterized TCEAL family of proteins, as a direct downstream target of BRG1-containing mammalian SWI/SNF (mSWI/SNF) complexes and calcineurin signaling during myoblast differentiation. Analyses of previously published datasets showed that pharmacological inhibition of mSWI/SNF bromodomains or knockdown of the BRG1 ATPase, but not knockdown of the homologue BRM ATPase, significantly reduced <i>Tceal7</i> expression in differentiating C2C12 myoblasts. We demonstrate that BRG1 occupancy at the <i>Tceal7</i> promoter increased during differentiation, paralleling the induction of <i>Tceal7</i> expression and nuclear accumulation of TCEAL7 protein. BRG1 functions in part by integrating calcium-dependent cues via the phosphatase calcineurin (Cn); we also determined that Cn knockdown or pharmacological inhibition of Cn suppressed <i>Tceal7</i> expression and impaired myoblast differentiation. The data suggest that both BRG1-driven chromatin remodeling and Cn signaling converge on <i>Tceal7</i> regulation. Functionally, <i>Tceal7</i> knockdown altered cell proliferation and disrupted myoblast differentiation, at least in part due to reduced expression of <i>Myogenin</i>, which encodes a transcription factor that is an essential differentiation determinant. RNA-seq analysis revealed broad dysregulation of myogenic, metabolic, and cell-cycle gene programs in <i>Tceal7</i>-deficient cells, including changes in cyclin-dependent kinase-regulated pathways consistent with prior reports linking TCEAL7 to cell-cycle control. Together, these findings identify TCEAL7 as a necessary component of the myogenic regulatory network whose expression is controlled by BRG1-dependent chromatin remodeling and Cn activity.</p></div> 2026-09-16T17:38:19Z Dataset Dataset 10.1371/journal.pone.0358038.s002 https://figshare.com/articles/dataset/_p_List_of_differentially_expressed_genes_DEGs_p_/33866376 CC BY 4.0