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oai:figshare.com:article/33857246 2026-09-16T15:04:35Z category_4 category_7 category_12 category_13 category_19 category_873 category_39 category_46 category_133 category_134 portal_63 item_type_3 month_year_09_2026

Cyclo(Val-Pro), a Potential Plant Immunomodulator, Confers Rice Blast Resistance via Circadian-Clock-Modulated Immune Activation and Metabolic Reprogramming Xinqing Wu (23039733) Nuo Cheng (9472327) Chenjie Yu (127596) Lin Song (270288) Mengyan Sun (7335500) Tao Lu (39406) Haifeng Qian (179918) Xiushi Song (2202934) Zhengwei Fu (179926) Yinhua Ni (307069) Biochemistry Medicine Cell Biology Genetics Pharmacology Chemical Sciences not elsewhere classified Ecology Immunology Plant Biology Virology small bioactive peptides potential plant immunomodulator modulated immune activation metabolomic analysis revealed low resistance risk friendly plant immunomodulators direct fungicidal activity activated salicylic acid circadian clock regulation jasmonic acid tryptophan metabolism sa )- rice leaves rewired phenylpropanoid promising eco metabolic reprogramming magnaporthe oryzae environmental risks callose deposition Rice blast, caused by Magnaporthe oryzae, is a devastating disease threatening global rice production, with current control strategies facing resistance and environmental risks. Small bioactive peptides have emerged as promising eco-friendly plant immunomodulators with low resistance risk for sustainable crop disease control. Here, we identified cyclic dipeptide cyclo(Val-Pro) as a potential plant immunomodulator with no direct fungicidal activity, which effectively conferred robust rice resistance to M. oryzae via promoting host intrinsic defense responses. Pre-treatment with cyclo(Val-Pro) activated salicylic acid (SA)- and jasmonic acid (JA)-mediated innate immunity, triggered reactive oxygen species (ROS) accumulation and callose deposition, and modulated core circadian clock genes in rice leaves. Transcriptomic and metabolomic analysis revealed that optimal blast resistance efficacy by cyclo(Val-Pro) pre-treatment at 14:00 was associated with circadian clock regulation and metabolic reprogramming. Specifically, cyclo(Val-Pro) rewired phenylpropanoid and tryptophan metabolism in rice leaves, and tryptamine was validated as a key functional downstream metabolite. 2026-09-16T00:00:00Z Dataset Dataset 10.1021/acs.jafc.6c07885.s001 https://figshare.com/articles/dataset/Cyclo_Val-Pro_a_Potential_Plant_Immunomodulator_Confers_Rice_Blast_Resistance_via_Circadian-Clock-Modulated_Immune_Activation_and_Metabolic_Reprogramming/33857246 CC BY-NC 4.0