2026-10-10T12:39:50Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33832988 2026-09-16T08:12:24Z category_4 category_146 category_8 category_272 category_873 category_39 category_734 category_69 portal_63 item_type_3 month_year_09_2026

Effect-Directed Toxicity Assessment of the Surface Water of Chao Phraya River by Integrating a Panel of Bioluminescent E. coli with ICP-OES and HPLC-DAD Calin Trif (24965483) Witthawat Piampowdee (24965486) Chiravoot Pechyen (16469407) Robert S. Marks (472227) Yardnapar Parcharoen (24965489) Biochemistry Space Science Microbiology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry tti ∼ 2 quorum sensing via performance liquid chromatography optical emission spectrometry new toxic index mechanistic biological diagnostics integrated framework demonstrates dynamic time warping diode array detection complex urban matrices chao phraya river total toxicity index monotonic toxicity masking directed toxicity assessment oes ), high overall toxicity directed analysis surface water study presents statistically associated phase extraction pathway stress oxidative stress metabolic disturbance km gradient kinetic alignment extreme salinity evaluating cytotoxicity dad ), bioluminescent e accurately evaluate 119 μg This study presents an effect-directed analysis (EDA) combining Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES), High-Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD), and five bioluminescent Escherichia coli bioreporters to map surface water toxicity along a 372-km gradient of the Chao Phraya River, Thailand. Evaluating cytotoxicity, genotoxicity, metabolic disturbance, oxidative stress, and quorum sensing via a new Toxic Index (TI) and Dynamic Time Warping (DTW) kinetic alignment, the panel unmasked complex cellular stress gradients. Although ICP-OES revealed localized mercury spikes (119 μg/L), metals contributed minimally to overall toxicity. Instead, upstream freshwater exhibited severe, multi-pathway stress (Total Toxicity Index, TTI ∼ 2.48) statistically associated with trace bioactive organic fractions isolated via solid-phase extraction. In complex urban matrices, DTW effectively exposed non-monotonic toxicity masking. Conversely, the downstream estuarine TTI peak (3.06) was identified as an isolated, non-chemical osmotic shock response driven by extreme salinity (18,710 ppm total dissolved solids). This integrated framework demonstrates that relying on bulk chemical quantification inherently underestimates latent ecotoxicological hazards, proving the necessity of mechanistic biological diagnostics to accurately evaluate and protect complex aquatic ecosystems. 2026-09-16T00:00:00Z Dataset Dataset 10.1021/acsestwater.6c00629.s001 https://figshare.com/articles/dataset/Effect-Directed_Toxicity_Assessment_of_the_Surface_Water_of_Chao_Phraya_River_by_Integrating_a_Panel_of_Bioluminescent_E_coli_with_ICP-OES_and_HPLC-DAD/33832988 CC BY-NC 4.0