2026-10-13T10:19:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33828385 2026-09-16T05:28:20Z category_8 portal_316 item_type_3 month_year_09_2026

Table 1_Genetic characterization of Listeria monocytogenes strains from the Seeliger’s historical “special Listeria culture collection” (1918–1986).xlsx Beatriz Daza Prieto (19272889) Ariane Pietzka (7446827) Johannes Benjamin Stielow (24960895) Anna Lennkh (24960898) Andrea Murer (24960901) David Eisele (23641567) Tobias Mösenbacher (20733677) Anna Stöger (701604) Patrick Hyden (8493912) Sven Halbedel (9287879) Franz Allerberger (511000) Herbert Hof (24960904) Werner Ruppitsch (701609) Microbiology antimicrobial resistance genes Listeria monocytogenes orthogroups virulence genes whole genome sequencing Introduction<p>Listeria monocytogenes (L. monocytogenes) is a widespread foodborne pathogen that continues to pose a major challenge to public health and the food industry.</p>Methods<p>To explore the genomic diversity of historical isolates, we used whole genome sequencing (WGS) both short- and long-read technologies to obtain high quality genomes of 64 historical L. monocytogenes isolates collected between 1918 and 1986. These isolates originated from environmental, animal, and human sources obtained from the collection from Professor Seeliger.</p>Results<p>The isolates displayed a substantial genetic diversity, representing 15 clonal complexes (CCs), 40 sequence types (STs) and 59 core genome multilocus sequence types (cgMLST). The most frequently identified STs were ST2 (n = 8), ST7 (n = 6) and ST9 (n = 4). All isolates harbored known virulence genes, while 35 isolates contained mobile genetic elements (MGEs), ranging from 1 to 11 per isolate. Ten isolates also carried a plasmid. Every isolate possessed the intrinsic resistance genes fosX and lin, and none carried acquired antimicrobial resistance genes (ARGs). Accessory genome diversity mainly followed phylogenetic positioning, and rendered limited within, but not between lineage variability.</p>Discussion/conclusion<p>WGS revealed high genetic diversity among the Seeliger L. monocytogenes isolates. Their close relatedness to contemporary isolates underscores the strong adaptability across sources and time, emphasizing the need for better prevention strategies.</p> 2026-09-16T05:28:20Z Dataset Dataset 10.3389/fmicb.2026.1909666.s002 https://figshare.com/articles/dataset/Table_1_Genetic_characterization_of_Listeria_monocytogenes_strains_from_the_Seeliger_s_historical_special_Listeria_culture_collection_1918_1986_xlsx/33828385 CC BY 4.0