2026-10-14T08:56:55Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33827767 2026-09-16T04:32:50Z category_393 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_MAFLD is linked to lower HBV DNA levels in chronic hepatitis B: clinical and transcriptomic insights.docx Xiaoyun Gao (2792791) Xuan Du (3692404) Foetal Development and Medicine chronic hepatitis B HBV DNA MAFLD metabolic heterogeneity transcriptomic analysis Background<p>Chronic hepatitis B (CHB) frequently coexists with metabolic dysfunction–associated fatty liver disease (MAFLD), yet the impact of MAFLD on hepatitis B virus (HBV) replication remains incompletely understood. We aimed to investigate the association between MAFLD and HBV DNA levels and to provide biological context through integrative transcriptomic analyses.</p>Methods<p>We analyzed a large retrospective cohort of patients with CHB and assessed the association between MAFLD and serum HBV DNA levels using linear regression models. HBV DNA was analyzed as a continuous outcome after log10 transformation. To provide molecular context for the clinical findings, weighted gene co-expression network analysis (WGCNA) was performed in an independent HBV liver transcriptomic dataset to identify HBV-associated host co-expression modules. These modules were subsequently characterized in independent liver transcriptomic datasets representing distinct metabolic and histological liver contexts using single-sample gene set enrichment analysis (ssGSEA).</p>Results<p>MAFLD was independently associated with lower circulating HBV DNA levels. This association varied across MAFLD phenotypes, with the lowest HBV DNA levels observed in overweight- or obesity-related MAFLD. Transcriptomic analyses identified HBV-associated host co-expression programs linked to lipid and cholesterol metabolism, inflammatory signaling, and antiviral responses. Cross-cohort analyses further showed that selected modules varied across distinct metabolic and fibrotic liver contexts.</p>Conclusions<p>MAFLD is associated with reduced HBV DNA levels in patients with CHB, especially in overweight- or obesity-related MAFLD. The transcriptomic findings provide complementary biological context for this clinical association and highlight metabolic and immune pathways that warrant further mechanistic investigation.</p> 2026-09-16T04:32:50Z Dataset Dataset 10.3389/fmed.2026.1926645.s001 https://figshare.com/articles/dataset/Data_Sheet_1_MAFLD_is_linked_to_lower_HBV_DNA_levels_in_chronic_hepatitis_B_clinical_and_transcriptomic_insights_docx/33827767 CC BY 4.0