2026-10-13T20:44:44Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33826663 2026-09-16T00:07:39Z category_1 category_4 category_146 category_915 category_7 category_12 category_19 category_21 category_873 category_64 portal_63 item_type_3 month_year_09_2026

Near-Infrared Organic Structures with Extended Lifetimes to Unlock the Potential of In Vivo Fluorescence Lifetime Imaging Elisa Chazeau (14844516) Hassan Al Sabea (24959044) Simone Janssen (16982142) Łukasz Mateusiak (21435797) Thomas Van den Dries (23142979) Thomas Lapauw (5210786) Sevada Sahakian (23142988) Marcus C.M. Stroet (24959047) Tim Dreessen (2650801) Maarten Kuijk (23142994) Catherine Paul (1585456) Hans Ingelberts (23142997) Sophie Hernot (849186) Anthony Romieu (27583) Christine Goze (1585465) Franck Denat (839548) Biophysics Biochemistry Space Science Physical Sciences not elsewhere classified Medicine Cell Biology Pharmacology Biotechnology Chemical Sciences not elsewhere classified Cancer short singlet excited infrared organic structures fluorescent contrast agents fluorescent antibody conjugates conjugation methods impact lifetime contrast allows spectrally overlapping fluorophores fluorescence lifetime imaging vivo </ italic fluorescence imaging longer lifetime lifetime differences lifetime characteristics fluorescence intensity vivo </ tumor imaging multiplexing imaging thereby expanding studies evaluating state lifetimes soluble nir physiological conditions microenvironmental variations irdye 800cw highly successful extended lifetimes enhanced sensitivity emissive aza bodipy dye <i>In vivo</i> fluorescence lifetime imaging (FLI) is an advanced optical imaging modality with the capability to reveal additional biological information compared to fluorescence intensity (FI) imaging. In addition to enabling tissue discrimination based on microenvironmental variations, lifetime contrast allows the differentiation of spectrally overlapping fluorophores, thereby expanding the high potential of multiplexing imaging. Until now, most of the studies evaluating the benefits of <i>in vivo</i> FLI in terms of enhanced sensitivity and specificity for tumor imaging with fluorescent contrast agents have been conducted using heptamethine cyanine dyes. While these fluorophores are highly successful for conventional <i>in vivo</i> fluorescence imaging, they are characterized by short singlet excited-state lifetimes (generally below 1 ns), making it challenging to achieve satisfying tissue contrast and to implement multiplexing imaging strategies based on their lifetime differences. Here, we report a bioconjugatable, water-soluble NIR-emissive aza-BODIPY dye with a 100% longer lifetime than IRDye 800CW in physiological conditions. We demonstrate its suitability for <i>in vivo</i> FLI when conjugated to cetuximab and reveal how conjugation methods impact the lifetime characteristics of fluorescent antibody conjugates. 2026-09-15T00:00:00Z Dataset Dataset 10.1021/acsomega.6c04430.s002 https://figshare.com/articles/dataset/Near-Infrared_Organic_Structures_with_Extended_Lifetimes_to_Unlock_the_Potential_of_In_Vivo_Fluorescence_Lifetime_Imaging/33826663 CC BY-NC 4.0