2026-10-12T01:34:55Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33820705 2026-09-15T19:57:43Z portal_1197 item_type_3 month_year_09_2026

Data for 3'-5' crosstalk contributes to transcriptional bursting Massimo Cavallaro (11015112) Mark David Walsh (24886186) Matt Jones (5830769) James Teahan (9695872) Simone Tiberi (10101607) Bärbel Finkenstädt (224917) Daniel Hebenstreit (760011) Gene regulatory networks -- Mathematical models Gene expression Monte Carlo method Bayesian statistical decision theory Migrated from ePrints Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to “transcriptional bursting” have been identified, usually classified as intrinsic, i.e., local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, each factor only accounts partially for the observed phenomenon, and some possible contributors have not been explored yet. We investigate processes at the 3’ and 5’ ends of a gene that enable reinitiation of transcription upon termination. Using an interdisciplinary approach, we measured the transcriptional bursting at a set of inducible transgenes with sufficient accuracy and precision to unveil significant patterns. We find that perturbation of polymerase shuttling typically reduces the average burst size and increases the burst frequency, thus limiting transcriptional noise. The observed noise patterns are reproduced by a generative model that captures major characteristics of the polymerase flux between a gene and a phase-separated compartment.<br><br>Data record consists of gzip archives and raw data in csv format. This repository contains supporting data to reference [1]. Please cite [1] if you find this repository useful. The data include: Flow cytometry data of HBB and HIV transgenes' expression in `.fcs` format. NanoString data for HIV expression. smFISH data for HBB and Akt1 gene expression. 2026-09-15T19:57:43Z Dataset Dataset 10.82444/warw.33820705.v1 https://figshare.com/articles/dataset/Data_for_3_-5_crosstalk_contributes_to_transcriptional_bursting/33820705 CC BY 4.0