2026-10-12T23:30:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33819106 2026-09-15T19:13:31Z category_1 category_4 category_8 category_272 category_39 category_734 category_133 category_135 portal_63 item_type_3 month_year_09_2026

Identification and Cyclization Mechanism of Fungal (+)-β-Chamigrene Synthase Yang Gao (18005) Li-Li Guo (6166310) Subiy Akbar (24952810) Zhiyuan Zhou (350818) Weiping Zhang (311523) Xinyang Li (1543561) He-Ping Chen (774696) Ji-Kai Liu (730885) Biophysics Biochemistry Microbiology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Plant Biology Computational Biology labeling experiments unveiled chamigrene synthase chamigranes cerrena albocinnamomea </ fungal (+)- β vitro </ sesquiterpene synthase (+)- β work provides unique class type terpenoids specifically catalyzes sesquiterpenoids featuring remained elusive proton shift forming rearrangement cyclization pathway cyclization mechanism cyclization followed biosynthetic production basidiomycetous fungus Chamigranes are a unique class of sesquiterpenoids featuring a spiro[5.5]­undecane framework, yet their biosynthesis in fungi has remained elusive for decades. In this study, we identify and characterize ChamTS, a sesquiterpene synthase from the basidiomycetous fungus <i>Cerrena albocinnamomea</i>, which specifically catalyzes the formation of (+)-β-chamigrane. <i>In vitro</i> isotope-labeling experiments unveiled the cyclization pathway of ChamTS, revealing an initial 1,6-cyclization followed by a 1,5-proton shift and spirocycle-forming rearrangement. This work provides a foundation for the biosynthetic production and further engineering of β-chamigrane-type terpenoids. 2026-09-25T00:00:00Z Dataset Dataset 10.1021/acs.orglett.6c03630.s001 https://figshare.com/articles/dataset/Identification_and_Cyclization_Mechanism_of_Fungal_-_-Chamigrene_Synthase/33819106 CC BY-NC 4.0