2026-10-14T01:35:03Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33810866 2026-09-15T17:34:24Z category_4 category_14 category_272 category_873 category_39 category_45 category_734 portal_5 item_type_3 month_year_09_2026

<p>Minimal datasets for dose response study.</p> Zeyede Teshome (24948290) Abebe Animut (845065) Belete Adefris Legesse (10902219) Mirutse Giday (25676) Esayas Aklilu (3911125) Biochemistry Molecular Biology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Sociology Biological Sciences not elsewhere classified regulatory performance standards natural insect repellents mitigate disease transmission major malaria vector human volunteers determined four medicinal plants eucalyptus globulus ,</ dermal toxicity assessments current study aimed botanical repellents provide protection duration demonstrating complete protection time anopheles arabiensis </ div >< p 131 chemical constituents seed oils provided mean percentage repellency juniperus procera </ echinops kebericho </ croton macrostachyus </ procera </ kebericho </ p </ personal protection macrostachyus </ chemical composition essential oils yet optimizing using hydrodistillation traditional means repellent efficacy reared mosquitoes oil underscores ms ). mass spectrometry laboratory conditions increasingly required identified across ghibe valley gas chromatography future investigations <div><p>Botanical repellents provide a traditional means of personal protection, yet optimizing their formulations is increasingly required to address shifting mosquito feeding behaviors and mitigate disease transmission. The current study aimed to evaluate the repellent efficacy of essential oils and their binary combinations as complex matrices against <i>Anopheles arabiensis</i> bites<i>,</i> the major malaria vector in Ethiopia. Essential oils were extracted from fresh parts of four ethnobotanically investigated medicinal plants in the Ghibe Valley, Ethiopia, namely <i>Croton macrostachyus</i>, <i>Echinops kebericho</i>, <i>Eucalyptus globulus,</i> and <i>Juniperus procera</i> using hydrodistillation. The chemical composition of the essential oils was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Arm-in-cage bioassays using laboratory-reared mosquitoes and human volunteers determined the effective dosage and complete protection time of the essential oils at 10% and 20% concentrations. A total of 131 chemical constituents were identified across the four analyzed essential oils. The <i>C. macrostachyus</i> leaf and seed oils provided a significantly longer protection time (<i>p</i> < 0.001), reaching 180 minutes with a mean percentage repellency of 85% to 100%. The essential oil of <i>J. procera</i> blended with <i>C. macrostachyus</i> or <i>E. kebericho</i> exhibited significant synergistic repellency (<i>p</i> < 0.001). The protection duration demonstrating by <i>C. macrostachyus</i> oil underscores their potential to meet the regulatory performance standards for natural insect repellents. Future investigations should include larger sample sizes, dermal toxicity assessments and field validation to fully support the commercialization of these essential oils.</p></div> 2026-09-15T17:40:38Z Dataset Dataset 10.1371/journal.pone.0358040.s005 https://figshare.com/articles/dataset/_p_Minimal_datasets_for_dose_response_study_p_/33810866 CC BY 4.0