2026-10-14T06:30:09Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33810376 2026-09-15T17:31:25Z portal_1197 item_type_3 month_year_09_2026

Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants Kathryn Murray (1332630) Ruben M. F. Tomás (24906688) Matthew I. Gibson (1273788) Cryopreservation of organs, tissues, etc. Stem cells -- Cryopreservation Biomedical materials Polymers Polyampholytes Flow cytometry Migrated from ePrints Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.<br><br>Data record consists of a zip archive containing flow cytometry and cell recovery details data, organised according to flow data and freezing data, and an accompanying index file. Flow and freezing data are in excel format. Original cell image files are too large to include here. 2026-09-15T17:31:25Z Dataset Dataset 10.82444/warw.33810376.v1 https://figshare.com/articles/dataset/Data_for_Low_DMSO_cryopreservation_of_stem_cells_enabled_by_macromolecular_cryoprotectants/33810376 CC BY 4.0