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Source data underlying the quantitative results presented in the manuscript. Jonathan Einterz Owen (24947971) Cheyanne Lynn Bemis (24947974) Qingyi Wang (4783575) Ambarish C. Varadan (24947977) Jacob W. Vander Velden (24947980) Laura Andačić (24947983) Olus Uyar (6570035) Mansi Gupta (485264) Christopher D. Scharer (8787563) Laurent Chatel-Chaix (59019) Pietro Scaturro (825718) Mehul S. Suthar (11349011) Christopher J. Neufeldt (10104405) Microbiology Cell Biology Genetics Molecular Biology Science Policy Immunology Biological Sciences not elsewhere classified Cancer Infectious Diseases Plant Biology Virology mutational analysis showed membrane rearrangements required innate immune responses host endoplasmic reticulum vro spatial distribution vro distribution corresponded confined within virus human primary cells membrane tethering plays mediated membrane organization known flaviviruses replicate div >< p defective atl2 mutant atl2 depletion leads limit flavivirus replication vro biogenesis virus production knockout cells infected cells zikv replication genome replication facilitate replication vros ). vro formation using confocal taken together robust induction rescue denv provide evidence possible avenue orthoflavivirus </ organize membranes flavivirus infection er ), electron microscopy conserved role central role borne viruses atl2 accumulates atl2 ), also demonstrate Excel workbook containing the individual numerical values underlying the graphs and statistical analyses in the main and supplementary figures. (XLSX) 2026-09-15T17:26:53Z Dataset Dataset 10.1371/journal.pbio.3003556.s012 https://figshare.com/articles/dataset/_p_Source_data_underlying_the_quantitative_results_presented_in_the_manuscript_p_/33809956 CC BY 4.0