2026-10-12T09:55:18Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33809939 2026-09-15T17:27:06Z category_1 category_4 category_12 category_13 category_14 category_873 category_46 category_64 category_122 category_132 category_134 category_135 portal_5 item_type_3 month_year_09_2026

<p>List of primers.</p> Irene Guzmán-Benito (24948020) Carmen Robinson (24948023) Livia Donaire (195357) Lourdes Fernández-Calvino (24948026) José Manuel Franco-Zorrilla (24948029) Ruixia Niu (15204961) Guoyong Xu (433581) Catharina Merchante (8219490) César Llave (152160) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Immunology Cancer Mental Health Infectious Diseases Virology Computational Biology responsive long non responsive gene expression multilayered regulatory mechanism div >< p 5 ’- leader impairing transcript cleavage directed transcript cleavage rna silencing mutants virus infection reduces identified another virus like kinase 1 directed mrna cleavage associated cleavage site mediated translational control mediated cleavage translational control like kinase coding rna potentially mediated virus infections transcriptional degradation repressive role plant immunity next demonstrate negative regulator natural antisense integrates srna findings reveal earlier described degradome analysis data reveal coding sequence coding region cis </ cell death broader implications brassinosteroid insensitive1 bak1 )< at4g39838 </ <div><p>In Arabidopsis, BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)<i>-</i>INTERACTING RECEPTOR-LIKE KINASE 1 (BIR1) is a negative regulator of plant immunity and cell death. <i>BIR1</i> was earlier described as a target of epigenetic and post-transcriptional degradation. During virus infections, degradome analysis of <i>BIR1</i> transcripts mapped predominant mRNA cleavage sites at the 5’-untranslated leader region (site A) and the protein-coding sequence (sites B and C). Here, we identified another virus-associated cleavage site (D) within the <i>BIR1</i> coding region and investigated the contribution of site-directed mRNA cleavage to <i>BIR1</i> regulation. Mutations at B, C, and D sites enhanced mRNA stability by impairing transcript cleavage, resulting in increased <i>BIR1</i> mRNA and protein accumulation. This regulation is disrupted in RNA silencing mutants, supporting a model of <i>cis</i>-directed small interfering RNA (siRNA)-mediated degradation. We next demonstrate that virus infection reduces <i>BIR1</i> translation in Arabidopsis. Furthermore, our data reveal a repressive role for the 5’-leader in regulating <i>BIR1</i> translation, potentially mediated by upstream open reading frames (uORFs) and a virus-responsive long non-coding RNA (lncRNA) derived from the natural antisense <i>At4g39838</i> locus. Together, these findings reveal a multilayered regulatory mechanism that integrates sRNA-mediated cleavage with translational control, with broader implications for the fine-tuning of stress-responsive gene expression during infection.</p></div> 2026-09-15T17:26:57Z Dataset Dataset 10.1371/journal.ppat.1014571.s006 https://figshare.com/articles/dataset/_p_List_of_primers_p_/33809939 CC BY 4.0