2026-10-14T06:21:59Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/33809237
2026-09-15T17:22:27Z
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<p>Figures Dataset.</p>
Erick J. Martínez Rodríguez (24947696)
P. Larry Phelan (24947699)
Luis Canas (24947702)
Nuris Acosta (4843077)
Paola N. Feliciano (24947705)
Jafina Tubbs (24947708)
Eric Loperena (24947711)
Harinantenaina L. Rakotondraibe (8496273)
Peter M. Piermarini (8496270)
Biochemistry
Cell Biology
Molecular Biology
Geology
Chemical Sciences not elsewhere classified
Ecology
Biological Sciences not elsewhere classified
Cancer
Science Policy
Infectious Diseases
Plant Biology
Virology
primary larvicidal component
div >< p
>) derived products
13 &# 181
quantify feeding deterrence
hemp leaf extracts
elicit spatial repellency
cannabis sativa </
assess spatial repellency
500 nl application
yellow fever mosquito
suggesting low volatility
higher equivalent doses
choice membrane blood
acute topical assay
aedes aegypti </
feeding repellency assay
hemp leaf extract
feeding assay
topical application
membrane blood
equivalent doses
aegypti </
hemp extract
hemp (<
taken together
results demonstrate
repellent effects
repellent compounds
recently demonstrated
potential sources
novel biopesticides
mosquitoes received
mart assay
fully explain
dependent manner
compounds contribute
assessed using
24 h
<div><p>Hemp (<i>Cannabis sativa</i>) derived products have emerged as potential sources of novel biopesticides. We recently demonstrated that a hemp leaf extract was toxic to larvae of the yellow fever mosquito <i>Aedes aegypti</i>, and that cannabidiol (CBD) was the primary larvicidal component of the extract. Here we evaluated the toxic and repellent effects of a hemp leaf extract and CBD against adult female <i>Ae. aegypti</i>. Toxicity was assessed using an acute topical assay in which mosquitoes received a 500 nL application to the thorax and mortality was recorded at 24 h. Repellency was then evaluated using two complementary bioassays: (i) a no-choice membrane blood-feeding assay to quantify feeding deterrence, and (ii) a mosquito airborne repellency test (MART) to assess spatial repellency. Topical application of CBD-rich hemp leaf extract induced dose-dependent mortality in adult <i>Ae. aegypti</i> with a LD<sub>50</sub> of 22.13 µg/mosquito at 24 h. When CBD isolate was tested at its equivalent doses in the extract, it caused significantly lower mortality than the hemp extract. In the membrane blood-feeding repellency assay, the hemp leaf extract repelled mosquitoes in a dose-dependent manner, whereas CBD produced weaker effects even at higher equivalent doses. In the MART assay, the hemp extract did not elicit spatial repellency, suggesting low volatility of the repellent compounds. Taken together, our results demonstrate that CBD does not fully explain the adulticidal or repellent effects of the CBD-rich hemp leaf extract against adult female <i>Ae. aegypti</i> suggesting that other compounds contribute to the bioactivity of hemp leaf extracts against adult female mosquitoes.</p></div>
2026-09-15T17:22:25Z
Dataset
Dataset
10.1371/journal.pone.0354696.s001
https://figshare.com/articles/dataset/_p_Figures_Dataset_p_/33809237
CC BY 4.0