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Data for Sex- and gamete-specific patterns of X chromosome segregation in a trioecious nematode André Pires da Silva (24893626) Nematodes -- Reproduction Nematodes -- Genetics Intersexuality in animals Meiosis Gametes Spermatogenesis Chromosomes Migrated from ePrints Three key steps in meiosis allow diploid organisms to produce haploid gametes: (1) homologous chromosomes (homologs) pair and undergo crossovers; (2) homologs segregate to opposite poles; and (3) sister chromatids segregate to opposite poles. The XX/XO sex determination system found in many nematodes [1] facilitates the study of meiosis because variation is easily recognized [2, 3, 4]. Here we show that meiotic segregation of X chromosomes in the trioecious nematode Auanema rhodensis [5] varies according to sex (hermaphrodite, female, or male) and type of gametogenesis (oogenesis or spermatogenesis). In this species, XO males exclusively produce X-bearing sperm [6, 7]. The unpaired X precociously separates into sister chromatids, which co-segregate with the autosome set to generate a functional haplo-X sperm. The other set of autosomes is discarded into a residual body. Here we explore the X chromosome behavior in female and hermaphrodite meioses. Whereas X chromosomes segregate following the canonical pattern during XX female oogenesis to yield haplo-X oocytes, during XX hermaphrodite oogenesis they segregate to the first polar body to yield nullo-X oocytes. Thus, crosses between XX hermaphrodites and males yield exclusively male progeny. During hermaphrodite spermatogenesis, the sister chromatids of the X chromosomes separate during meiosis I, and homologous X chromatids segregate to the functional sperm to create diplo-X sperm. Given these intra-species, intra-individual, and intra-gametogenesis variations in the meiotic program, A. rhodensis is an ideal model for studying the plasticity of meiosis and how it can be modulated.<br><br>Data record consists of a single data file in .xlsx format. This excel document contains information for the genotyping of 5 X chromosome markers and 5 autosomal (LG4) markers of the nematode Auanema rhodensis. Each marker contains a Single Nucleotide Polymorphism (SNP) between two strains of A. rhodensis (APS4 and APS6). This SNP creates a restriction enzyme cut site in one strain but not the other. Genotyping of the markers is performed by PCR amplification followed by digestion with a restriction enzyme. This spread sheet includes, for each marker, the full marker sequence, the position and nucleotide difference characterizing the SNP, the forward and reverse primers used to amplify the marker, the restriction enzyme used to differentiate one strain from the other, the strain being cut and the undigested and digested PCR product sizes. 2017-11-14T00:00:00Z Dataset Dataset 10.82444/warw.33802636.v1 https://figshare.com/articles/dataset/Data_for_Sex-_and_gamete-specific_patterns_of_X_chromosome_segregation_in_a_trioecious_nematode/33802636 CC BY 4.0