2026-10-08T14:01:49Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33801790 2026-09-15T15:07:29Z category_133 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Structural and functional perspectives on DEAD-box RNA helicases in the rubber tree cold stress response.zip Baocai Gao (8160792) Mingxia Xiao (24942118) Changli Mao (14368764) Jin Liu (12833) Plant Biology AlphaFold2 cold stress DExD/H-box family RNA helicases rubber tree structural biology <p>DExD/H-box RNA helicases constitute one of the largest families of adenosine triphosphate (ATP)-dependent nucleic-acid enzymes, with roles spanning the full breadth of RNA metabolism, from transcription to turnover. Their defining architectural feature is a conserved helicase core comprising two tandem RecA-like domains that couples ATP hydrolysis to duplex unwinding and ribonucleoprotein remodeling. Structural investigations have historically focused on human and yeast model systems, but recent methodological advances, particularly cryo-electron microscopy and deep learning-based structure prediction, have broadened the structural coverage of the DDX family, including full-length constructs and their incorporation into multiprotein assemblies. In plants, DDX helicases remain far less characterized despite their essential functions in development and stress adaptation. This review integrates structural biology insights with recent multi-omics datasets from cold-stressed rubber tree (Hevea brasiliensis), a tropical crop highly susceptible to chilling injury. Quantitative profiling has identified nine differentially abundant DDX proteins under cold stress, among which DDX39B shows the most pronounced response. Rubber tree DDX39B shares >95% sequence identity with orthologs from other tropical species and ~73% identity with human DDX39B; AlphaFold2 predictions reveal near-identical core helicase folds, consistent with strong functional constraint across vast evolutionary distances. These predictions also reveal plant-specific domain arrangements, including a putative DDX39B isoform bearing additional LysM and protein kinase domains. This work illustrates how computational structure prediction can bridge the sequence-to-function gap for stress-responsive proteins in non-model plant species and provides a conceptual framework for dissecting DDX helicase roles in plant abiotic stress tolerance.</p> 2026-09-15T15:07:29Z Dataset Dataset 10.3389/fpls.2026.1959829.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Structural_and_functional_perspectives_on_DEAD-box_RNA_helicases_in_the_rubber_tree_cold_stress_response_zip/33801790 CC BY 4.0