2026-10-12T21:10:19Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33790840 2026-09-15T11:59:53Z portal_1197 item_type_3 month_year_09_2026

Data for Synthetically scalable poly(ampholyte) which dramatically enhances cellular cryopreservation Trisha L. Bailey (5843498) Christopher Stubbs (3601565) Kathryn Murray (1332630) Ruben M. F. Tomás (24906688) Lucienne Otten (6910733) Matthew I. Gibson (1273788) Cryopreservation of organs, tissues, etc. Cells -- Cryopreservation Frozen erythrocytes Polyampholytes Migrated from ePrints The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-dimensional (2D) or three-dimensional (3D) cell models, and the solvent and cell debris must be removed prior to application/transfusion. There is a real need to improve this 50-year-old method to underpin emerging regenerative and cell-based therapies. Here, we introduce a potent and synthetically scalable polymeric cryopreservation enhancer which is easily obtained in a single step from a low cost and biocompatible precursor, poly(methyl vinyl ether-alt-maleic anhydride). This poly(ampholyte) enables post-thaw recoveries of up to 88% for a 2D cell monolayer model compared to just 24% using conventional DMSO cryopreservation. The poly(ampholyte) also enables reduction of [DMSO] from 10 wt % to just 2.5 wt % in suspension cryopreservation, which can reduce the negative side effects and speed up post-thaw processing. After thawing, the cells have reduced membrane damage and faster growth rates compared to those without the polymer. The polymer appears to function by a unique extracellular mechanism by stabilization of the cell membrane, rather than by modulation of ice formation and growth. This new macromolecular cryoprotectant will find applications across basic and translational biomedical science and may improve the cold chain for cell-based therapies.<br><br>Differential scanning calorimetry includes data used to calculate ice crystallization enthalpy and melting temperature, along with full DSC traces and is located in the “DSC” folder. Ice recrystallization data was used to calculate the mean grain size is located in the “IRI” folder. Cytotoxicity data was used to calculate alamar blue reduction for P2 and DMSO and each file is located in the “mammalian cell cytotoxicity” folder. Monolayer and suspension recoveries for cryopreserved A549 is located in the “mammalian cell freezing” folder, subfolder “A549 cell freezing” and includes P2 concentration recoveries, lower DMSO concentrations with P2, and molecular weight effects of polyampholyte. Monolayer recoveries for cryopreserved MC-3T3 and Neuro-2a cells is located in the “mammalian cell freezing” folder, subfolder “Neuro-2a & MC-3T3 cell freezing”. Fold change data for all three cell lines can be found in the “mammalian cell freezing” folder. Post-freeze A549 growth rate data is located in the “mammalian cell growth rates” folder. Membrane kinetics for incubated A549 cells is located in the “mammalian cell membrane kinetics” folder. Live/dead graphical representation from post-freeze staining data is located in the “mammalian cell post-freeze imaging” folder. Polyampholyte NMR and FITR data is located in the “polymer characterisation” folder. Red blood cell incubation results, osmotic fragility, polyampholyte freezing, and polymer comparisons data is located in the “red blood cell freezing” folder. 2019-07-31T00:00:00Z Dataset Dataset 10.1021/acs.biomac.9b00681 https://figshare.com/articles/dataset/Data_for_Synthetically_scalable_poly_ampholyte_which_dramatically_enhances_cellular_cryopreservation/33790840 CC BY 4.0