2026-10-10T03:02:04Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/33782642
2026-09-15T09:38:35Z
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month_year_09_2026
Enhanced antibacterial and antibiofilm activity of <i>Artemisia aucheri</i> Boiss. extract-loaded niosomes against clinical MRSA isolates
Fatemeh Ashrafi (2626663)
Mahtab Majdabadi (24923330)
Pooria Moulavi (24923333)
Sepideh Asadi (24923336)
Sadegh Jozaie (24923339)
Hojjatollah Shokri (24923342)
Biochemistry
Space Science
Medicine
Microbiology
Cell Biology
Molecular Biology
Pharmacology
Biotechnology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Developmental Biology
Cancer
Infectious Diseases
Virology
Artemisia aucheri
antibacterial
biofilm
Staphylococcus aureus
niosome
<p>To develop and characterize niosomes encapsulating ethanolic <i>Artemisia aucheri</i> extract (Nio-Artemisia) and evaluate their antibacterial and antibiofilm activities against clinical methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolates.</p> <p>Niosomes were prepared by thin-film hydration and optimized based on particle size, polydispersity index (PDI), and encapsulation efficiency (EE). Stability and <i>in vitro</i> release were evaluated. Clinical <i>S. aureus</i> isolates were identified using standard microbiological methods, and antibacterial activity was assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and growth kinetics. Antibiofilm activity and expression of <i>icaA</i> and <i>icaB</i> were evaluated using the crystal violet assay and quantitative real-time PCR, respectively.</p> <p>The optimized formulation had a particle size of 230.2 ± 9.1 nm, PDI of 0.191 ± 0.020, and EE of 72.39 ± 1.45%. Release followed a biphasic pattern and fitted the Higuchi model. Of 30 clinical S. aureus isolates, 15 were MRSA. Niosomal encapsulation reduced MIC values 4- to 16-fold compared with free extract and significantly inhibited biofilm formation. icaA and icaB expression was significantly downregulated (<i>p</i> < 0.001).</p> <p>Niosomal encapsulation enhanced the antibacterial and antibiofilm activities of <i>A. aucheri</i> extract against clinical MRSA isolates.</p>
2026-09-15T09:38:35Z
Dataset
Dataset
10.6084/m9.figshare.33782642.v1
https://figshare.com/articles/dataset/Enhanced_antibacterial_and_antibiofilm_activity_of_i_Artemisia_aucheri_i_Boiss_extract-loaded_niosomes_against_clinical_MRSA_isolates/33782642
CC BY 4.0