2026-10-14T06:29:47Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33780400 2026-09-15T09:01:01Z portal_1197 item_type_3 month_year_09_2026

Data for Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles Laura E. Wilkins (4688752) Muhammad Hasan (5226662) Alice E. R. Fayter (5122613) Caroline I. Biggs (1365108) Marc Walker (1429663) Matthew I. Gibson (1273788) Antifreeze proteins Nanoparticles Ice crystals -- Growth Migrated from ePrints Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent ‘antifreeze’ active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential. This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.<br><br>Background data is contained in Excel or original format. This dataset contains: - Nanoparticle and polymer characterisation data (DLS, SEC) - Ice growth data from the ‘splat’ assay 2019-06-05T00:00:00Z Dataset Dataset 10.1039/c8py01719k https://figshare.com/articles/dataset/Data_for_Site-specific_conjugation_of_antifreeze_proteins_onto_polymer-stabilized_nanoparticles/33780400 CC BY 4.0