2026-10-13T08:04:45Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33771769 2026-09-15T05:35:16Z category_696 portal_316 item_type_3 month_year_09_2026

Table 1_Sleep fragmentation frequency is associated with distinct immune, microbial, and barrier-related profiles in DSS-induced colitis in mice.docx Ming-Shih Tu (24912574) Ting-An Fan (24912577) Yueh-Hsiang Huang (5419274) Ren-Chin Wu (167828) Chia-Jung Kuo (1525771) Ning-Hung Chen (291356) Li-Pang Chuang (447777) Shih-Wei Lin (447775) Yen-Lung Chen (447776) Huang-Yu Yang (166482) Geng-Hao Liu (5419289) Genetic Immunology epithelial barrier gut microbiota microbiota-derived metabolites short-chain fatty acids sleep fragmentation ulcerative colitis Background<p>Sleep fragmentation is common in inflammatory bowel disease, but whether different fragmentation frequencies are associated with distinct biological responses during colitis remains unclear. This study examined endpoint-specific responses to low- and high-frequency sleep fragmentation in DSS-induced colitis in mice.</p>Methods<p>Male C57BL/6 mice were assigned to Control, DSS, DSS with low-frequency sleep fragmentation (DSS+LSF), DSS with high-frequency sleep fragmentation (DSS+HSF), or high-frequency sleep fragmentation alone (HSF). Colitis was induced with 2% DSS for 7 days. Sleep fragmentation was applied for 12 h/day during the light phase at 60 sweeps/h for LSF or 240 sweeps/h for HSF. Clinical indices, histology, epithelial barrier-associated proteins, inflammatory and stress-related markers, colonic CD4⁺ T-cell subsets, fecal microbiota composition, and fecal SCFAs were assessed.</p>Results<p>Clinical and macroscopic indices varied across experimental groups, but corrected comparisons among DSS-treated groups were not significant. Plasma corticosterone was increased in both DSS+LSF and DSS+HSF, whereas MPO was higher in DSS+LSF than in DSS+HSF. Colonic Th2 proportions were higher in DSS+HSF than in DSS and DSS+LSF. Other histological, barrier-associated, inflammatory, and metabolic findings were primarily descriptive.</p>Conclusions<p>Low- and high-frequency sleep fragmentation were associated with distinct, endpoint-specific response profiles during DSS-induced colitis rather than a simple linear increase in disease severity. These findings support frequency-associated, domain-specific responses and warrant further longitudinal and mechanistic investigation.</p> 2026-09-15T05:35:16Z Dataset Dataset 10.3389/fimmu.2026.1885856.s001 https://figshare.com/articles/dataset/Table_1_Sleep_fragmentation_frequency_is_associated_with_distinct_immune_microbial_and_barrier-related_profiles_in_DSS-induced_colitis_in_mice_docx/33771769 CC BY 4.0