2026-10-14T02:46:07Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33770917 2026-09-15T04:28:05Z category_21 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_A multifunctional Fe3O4@Au-HPG-Glc theranostic platform for MRI/fluorescence-guided photothermal ablation of osteosarcoma.docx Shengren Xiong (22633154) Xiaomei Zheng (2531440) Zhe Wu (582498) Shenghui Wu (2602330) Zhongliang Yao (24912061) Weilin Zheng (7513565) Biotechnology Fe3O4@Au-HPG-Glc in vivo Nanotecehnology osteosarcoma photothermal therapy Background<p>The clinical management of osteosarcoma is challenged by its aggressive growth and deep anatomical location. Photothermal therapy offers a minimally invasive approach through localized thermal ablation, but its effectiveness is constrained by poor tissue penetration and inaccurate targeting of deep tumors without real-time guidance.</p>Methods<p>We synthesized flower-like Fe₃O₄@Au nanoparticles coated with hyperbranched polyglycerol (HPG) and conjugated with glucose (Glc) (Fe₃O₄@Au-HPG-Glc). In an osteosarcoma mouse model, the photothermal efficacy of the nanoprobes was evaluated by comparing two groups: the control group receiving phosphate-buffered saline plus laser irradiation (phosphate-buffered saline (PBS) + Laser) and the treatment group receiving Fe₃O₄@Au-HPG-Glc nanoprobes plus laser irradiation (NPs + Laser). Intratumoral temperature was monitored by infrared thermography, with longitudinal tracking of tumor volume and body weight. Major organs were examined by H&E staining. Tumor accumulation was assessed via whole-body fluorescence and T₂-weighted magnetic resonance imaging (MRI); pharmacokinetics and biodistribution were quantified by inductively coupled plasma mass spectrometry (ICP-MS).</p>Results<p>The NPs + Laser regimen produced rapid intratumoral heating during the 0–4 min irradiation window and significantly delayed tumor growth compared with the PBS + Laser control group, with stable body weight and no overt organ lesions on H&E staining. Whole-body fluorescence and T₂-weighted MRI confirmed tumor-site accumulation of the nanoprobes. ICP-MS-based pharmacokinetic and biodistribution analyses provided quantitative exposure context, including systemic clearance, reticuloendothelial system capture, and measurable tumor uptake. Together, these data facilitated interpretation of the subsequent photothermal response.</p>Discussion<p>Flower-like Fe₃O₄@Au-HPG-Glc enables image-guided photothermal treatment in an osteosarcoma model, supported by quantitative pharmacokinetic and biodistribution measurements and an acceptable short-course safety profile. This work establishes a clear imaging-exposure-response relationship and provides a strong foundation for further optimization of magnetic-optical combination strategies in precision cancer therapy.</p> 2026-09-15T04:28:05Z Dataset Dataset 10.3389/fbioe.2026.1868939.s001 https://figshare.com/articles/dataset/Supplementary_file_1_A_multifunctional_Fe3O4_Au-HPG-Glc_theranostic_platform_for_MRI_fluorescence-guided_photothermal_ablation_of_osteosarcoma_docx/33770917 CC BY 4.0