2026-10-14T21:48:53Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33769771 2026-09-15T06:20:50Z category_24184 item_type_3 month_year_09_2026

HG002 Gradient Test zheng shang (24756460) Bioinformatic methods development HG002 human broken-reference benchmark <p dir="ltr">To systematically evaluate the performance of various chromatin conformation capture technologies and 3D scaffolding algorithms at the telomere-to-telomere (T2T) level, we utilized the published high-quality Hg002(mat) T2T reference genome (https://doi.org/10.1016/j.cell.2026.06.016) as the gold standard. The original T2T genome was computationally fragmented into simulated contigs with three length gradients: 1 Mb, 2 Mb, and 5 Mb. Utilizing the publicly available human HiFi-C sequencing dataset (https://www.ncbi.nlm.nih.gov/sra/ERR14275147), chromosome-level scaffolding was independently performed using three tools: wf-pore-C, CPhasing, and 3D-HiFi. Finally, the resulting scaffolds were aligned against the original T2T reference genome via whole-genome alignment to rigorously assess and validate the scaffolding accuracy and robustness across different contig continuities.</p> 2026-09-15T06:20:50Z Dataset Dataset 10.6084/m9.figshare.33769771.v1 https://figshare.com/articles/dataset/HG002_Gradient_Test/33769771 MIT