2026-10-13T02:14:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33756190 2026-09-14T20:08:38Z category_4 category_915 category_8 category_12 category_13 category_15 category_16 category_873 category_46 category_734 category_69 portal_63 item_type_3 month_year_09_2026

Integrated Proteomic Profiling of Inner Ear and Systemic Fluids Following Noise-Induced Hearing Loss in Rats Motahare Khorrami (24899209) Zeshan Ali (9682315) Robert D. Gay (24899212) Ya Lang Enke (24899215) Mohsen Asadnia (4040633) Paul A. Haynes (1538398) Christopher Pastras (7961612) Biochemistry Physical Sciences not elsewhere classified Microbiology Cell Biology Genetics Neuroscience Physiology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Inorganic Chemistry shared inflammatory response integrated proteomic profiling induced hearing loss identify molecular signatures derived cerebrospinal fluid based proteomic analysis identified 310 proteins 110 db noise early nihl detection related proteins noise trauma systemic responses study presents standard audiometry regulatory pathways prolonged exposure peripheral biomarkers mode followed loud sounds level filtering inner ear independent acquisition including pgam2 ighm across fibrinogen chains consistent upregulation cap ). 1 h Noise-induced hearing loss (NIHL) arises from acute or prolonged exposure to loud sounds, yet early diagnosis remains difficult as some individuals show minimal changes in standard audiometry. This study presents the first comparative mass spectrometry-based proteomic analysis of perilymph, cochlear-derived cerebrospinal fluid (CSF), whole blood, and plasma in Sprague–Dawley rats following acute noise exposure, to identify molecular signatures of NIHL. Rats were exposed to 110 dB noise for 1 h under anesthesia, inducing an approximately 40 dB threshold shift measured by cochlear nerve Compound Action Potential (CAP). Nano LC–MS/MS in data-independent acquisition (DIA) mode followed by peptide- and protein-level filtering, identified 310 proteins in perilymph, 396 in CSF, 1688 in blood, and 281 in plasma. Differential expression analysis revealed 50, 79, 281, and 49 significantly changed proteins, respectively. A shared inflammatory response was observed, highlighted by consistent upregulation of IGHM across all fluids. Cochlear samples showed enrichment of metabolic and injury-related proteins, including PGAM2 and ALDOA, while systemic samples exhibited changes in fibrinogen chains, IGFBP5, and apolipoproteins. KEGG pathway analysis indicated involvement of metabolic, immune, and lipid-regulatory pathways. Targeted PRM validation confirmed selected protein changes. These findings demonstrate coordinated local and systemic responses to noise trauma, supporting the potential of peripheral biomarkers for early NIHL detection. 2026-09-14T00:00:00Z Dataset Dataset 10.1021/acs.jproteome.6c00316.s001 https://figshare.com/articles/dataset/Integrated_Proteomic_Profiling_of_Inner_Ear_and_Systemic_Fluids_Following_Noise-Induced_Hearing_Loss_in_Rats/33756190 CC BY-NC 4.0