2026-10-14T14:17:17Z
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2026-09-14T14:01:21Z
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Potential nephroprotective effects of the Chinese herb <i>Angelica sinensis</i> against cisplatin tubulotoxicity
Valérian Bunel (24886929)
Marie-Hélène Antoine (2675737)
Joëlle Nortier (441926)
Pierre Duez (475090)
Caroline Stévigny (771633)
Microbiology
Cell Biology
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Cancer
Hematology
β-Catenin
apoptosis
collagen
HK-2 cells
Ki-67
nephroprotection
oxidative stress
regeneration
<p><i>Context</i>: Acute kidney injury (AKI) is often encountered in patients receiving cisplatin (CisPt), a chemotherapeutic drug that induces numerous toxic side effects. Techniques used to limit nephrotoxicity during CisPt treatment are not fully effective; about a third of patients experience AKI. New nephroprotective strategies, including pharmacological approaches, must be developed.</p> <p><i>Objective</i>: The present study investigated the nephroprotective potential of <i>Angelica sinensis</i> (Oliv.) Diels (Apiaceae) root towards CisPt tubulotoxicity.</p> <p><i>Materials and methods</i>: HK-2 cells were incubated with CisPt (10 µM) and/or with a methanolic extract of <i>A. sinensis</i> (AS). Nephroprotective capacity was evaluated by means of cellular viability (resazurin assay) and apoptosis (annexin-V/PI staining), oxidative stress generation (H<sub>2</sub>DCF-DA oxidation), Ki-67 index (immunofluorescence), cell cycle analysis (DNA staining), cell migration rate (scratch assay), extracellular matrix deposition (collagen determination), and β-catenin relocalization.</p> <p><i>Results</i>: CisPt decreased cell viability [76% versus Ctrl], which was associated with an increased apoptosis. Simultaneous treatment with 50 µg/ml AS enhanced cell survival [84% versus Ctrl] and decreased the apoptosis rate. AS could not alleviate CisPt-induced oxidative stress; but doses of 5 and 50 µg/ml raised the Ki-67 index [135 and 244% versus Ctrl] and cell migration rates [1.2 and 1.3-fold versus Ctrl]. Finally, both doses of AS limited the amount of collagen deposition [121.6 and 119.6% for 5 and 50 µg/ml, respectively, versus 131.0% for CisPt-treated cells] and prevented the relocalization of β-catenin from the membrane to the nucleus.</p> <p><i>Conclusion</i>: These results confirm the nephroprotective potential of <i>A. sinensis</i> and require further investigations aiming at identifying its active compounds.</p>
2026-09-14T14:01:21Z
Dataset
Dataset
10.6084/m9.figshare.33741981.v1
https://figshare.com/articles/dataset/Potential_nephroprotective_effects_of_the_Chinese_herb_i_Angelica_sinensis_i_against_cisplatin_tubulotoxicity/33741981
CC BY 4.0