2026-10-12T14:51:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33737572 2026-09-14T12:43:56Z category_133 portal_316 item_type_3 month_year_09_2026

Supplementary file 2_Uncovering natural variation in TdBCAT genes and phenotypic variation under drought in durum wheat.docx Valentina Buffagni (9083423) Donatella Danzi (703104) Ricardo Humberto Ramirez-Gonzalez (24883498) Cristobal Uauy (779399) Mariolina Gullì (693195) James Simmonds (702706) Angelo Petrozza (6261848) Francesco Cellini (736176) Stephan Summerer (6261851) Domenico Pignone (5767754) Michela Janni (1614964) Nelson Marmiroli (693199) Plant Biology drought durum wheat germplasm genotype selection genotyping landraces phenotype selection phenotyping <p>Drought severely limits wheat productivity, highlighting the need for tolerant genetic resources. Targeted allele mining and high-throughput phenotyping (HTP) were combined to assess natural variation in TdBCAT genes and drought response in 38 durum wheat genotypes. A cost-effective strategy integrating next-generation sequencing and Kompetitive Allele Specific PCR (KASP) genotyping identified 27 single nucleotide polymorphisms (SNPs) and 10 haplotypes across TdBCAT-A and TdBCAT-B, mainly in promoter regions. Under controlled drought, RGB imaging and the digital biovolume ratio (DBR) classified SSD 195, SSD 269, SSD 322, SSD 343, SSD 409, SSD 416, SSD 441, and Svevo as the best-performing genotypes, with SSD 195 and SSD 441 showing the greatest stability. Integrating genotypic and phenotypic data identified TdBCAT haplotype combinations associated with improved drought performance and valuable landraces for pre-breeding. This integrated approach efficiently characterized haplotypic diversity and identified drought-adaptive germplasm, providing valuable resources for pre-breeding and the sustainable selection of durum wheat under water-limited conditions.</p> 2026-09-14T12:43:56Z Dataset Dataset 10.3389/fpls.2026.1826616.s001 https://figshare.com/articles/dataset/Supplementary_file_2_Uncovering_natural_variation_in_TdBCAT_genes_and_phenotypic_variation_under_drought_in_durum_wheat_docx/33737572 CC BY 4.0