2026-10-06T18:13:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33735580 2026-09-24T06:02:37Z category_23989 item_type_3 month_year_09_2026

Multi-omics insights into the metabolic basis of variation in 4-alkyl branched-chain fatty acids in meat from Chinese sheep breed Jinlong Li (20481890) Food chemistry and food sensory science 4-methyloctanoic acid Propanoate metabolism Branched-chain amino acids <p dir="ltr">4-Alkyl branched-chain fatty acids (BCFAs) contribute to characteristic mutton odor, but their variation among sheep breeds and underlying metabolic basis remain unclear. We quantified 4-methyloctanoic acid (4-MOA), 4-ethyloctanoic acid (4-EOA), and 4-methylnonanoic acid (4-MNA) and integrated muscle metabolomics with public multi-omics data to explore the metabolic basis of breed differences. BCFA deposition varied among breeds. The three target compounds were undetectable in Altay and Turpan sheep, whereas Hu and Tan sheep had detectable levels of at least two compounds. In Hu sheep, 4-MOA and 4-EOA concentrations were approximately 14.4 and 12.5 times those in Tan sheep, respectively (p < 0.001). Metabolomic and integrative analyses identified differences in amino acid, lipid, and energy metabolism, with branched-chain amino acid (BCAA) degradation, propanoate metabolism, and the tricarboxylic acid (TCA) cycle recurring across comparisons. Metabolic differences across breeds and feeding systems suggested that this BCAA degradation–propanoate metabolism–TCA cycle module may link carbon utilization and metabolic state to BCFA deposition. These findings characterize metabolic features associated with breed differences in odor-related BCFAs and identify a candidate network for further studies of mutton odor formation, selective breeding, and nutritional modulation.</p> 2026-09-24T06:02:37Z Dataset Dataset 10.6084/m9.figshare.33735580.v2 https://figshare.com/articles/dataset/Integrative_Analysis_of_Metabolomics_and_Public_Multi-Omics_Data_Reveals_Metabolic_Pathways_Associated_with_Breed_Differences_in_Mutton_Odor-Related_Branched-Chain_Fatty_Acids/33735580 CC BY 4.0