2026-10-07T10:18:25Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33735280 2026-09-14T12:45:12Z category_24415 item_type_3 month_year_09_2026

The sleep-promoting ventrolateral preoptic nucleus is organized into two functionally distinct compartments Leana Razaghi (24881008) Felix Bellier (24882166) Laure Lecoin (24882229) Nathalie Rouach (9086613) Armelle Rancillac (9086600) Animal neurobiology Neuron VLPO Sleep Electrophysiology LTS EPSC IPSC Hypothalamus Neuroanatomy <p dir="ltr">Whether the galaninergic core of the ventrolateral preoptic nucleus (VLPO), described only as a dense triangular cluster of sleep-promoting neurons, possesses an internal organization is unknown. </p><p dir="ltr">We asked whether it is spatially and functionally compartmentalized. In P30 Gal-GFP mice, euthanized at ZT4, confocal profiles of neuronal (Gal-GFP, NeuN), synaptic (VGAT, gephyrin), astrocytic (Sox9, Cx30), and activity (c-Fos) fluorescent markers were measured in the center of the VLPO, from the pia to 260 µm, and fitted with a two-segment piecewise linear model. </p><p dir="ltr">In the connected article, we found that all neuronal and astroglial markers decline with depth, with a mean inflection point at 98 ± 8 µm, defining a superficial Compartment I and a deeper Compartment II. Electrophysiological recordings further reveal compartment-specific differences in neuronal excitability and synaptic input, with intrinsic properties varying progressively with depth. Synaptic input shows a step-like transition between compartments. Spike-generation properties are conserved.</p><p dir="ltr">The linked set contains a data table of galaninergic fluorescence (Input_Matrix.xlsx) across distance (the pial surface to 206 µm) and the MATLAB script (fit_piecewise_linear_0.m) to run the piecewise fit model, as well as the script to generate the figure as % max intensity (run_real_data_GFP_center_max.m).</p><p dir="ltr"><br></p> 2026-09-14T12:45:12Z Dataset Dataset 10.6084/m9.figshare.33735280.v1 https://figshare.com/articles/dataset/The_sleep-promoting_ventrolateral_preoptic_nucleus_is_organized_into_two_functionally_distinct_compartments/33735280 CC BY 4.0