2026-10-12T04:49:40Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/33724955
2026-09-28T04:06:05Z
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month_year_09_2026
Functional evidence of Hv1-mediated proton flux in cardiac mitochondria
Rayen De Fazio (24872579)
Clara Ventura (24872582)
Paulina Finochietto (24872585)
Ernesto A. Aiello (12452412)
Carolina Jaquenod De Giusti (12452409)
Verónica Milesi (24872588)
Biophysics
Biochemistry
Cell Biology
Genetics
Neuroscience
Physiology
Pharmacology
Environmental Sciences not elsewhere classified
Developmental Biology
Cancer
Computational Biology
Hv1 channel
mitochondria
pH
membrane potential
heart
<p>Mitochondria are essential for cardiac myocyte function, providing the continuous ATP supply required for contraction and cellular homeostasis. Regulation of the proton motive force, which is critical for ATP synthesis, depends on multiple ion transport mechanisms, including those mediating H<sup>+</sup> flux. The voltage-gated proton channel (Hv1), encoded by the <i>Hvcn1</i> gene, is known to regulate cytosolic pH and membrane potential in several cell types. Here, using isolated mouse cardiac mitochondria, we demonstrate for the first time that Hv1 is functionally expressed in cardiac mitochondria. Pharmacological inhibition of Hv1 enhanced matrix alkalinization and induced mitochondrial hyperpolarization during succinate-driven mitochondrial energization. Importantly, a similar hyperpolarization was also observed under basal conditions in intact cardiac myocytes.</p>
2026-09-28T04:06:05Z
Dataset
Dataset
10.6084/m9.figshare.33724955.v2
https://figshare.com/articles/dataset/Functional_evidence_of_Hv1-mediated_proton_flux_in_cardiac_mitochondria/33724955
CC BY 4.0