2026-10-12T15:02:28Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33723265 2026-09-15T01:23:20Z category_24184 item_type_3 month_year_09_2026

Cotton Gradient Test zheng shang (24756460) Bioinformatic methods development cotton broken-reference benchmark <p dir="ltr">To systematically evaluate the performance of various chromatin conformation capture technologies and 3D scaffolding algorithms at the telomere-to-telomere (T2T) level, we utilized the published high-quality cotton T2T reference genome (https://www.nature.com/articles/s41588-025-02130-4) as the gold standard. The original T2T genome was computationally fragmented into simulated contigs with three length gradients: 1 Mb, 2 Mb, and 5 Mb. Utilizing the publicly available cotton Pore-C sequencing dataset (https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13877), chromosome-level scaffolding was independently performed using three tools: wf-pore-C, CPhasing, and 3D-HiFi. Finally, the resulting scaffolds were aligned against the original T2T reference genome via whole-genome alignment to rigorously assess and validate the scaffolding accuracy and robustness across different contig continuities.</p> 2026-09-15T01:23:20Z Dataset Dataset 10.6084/m9.figshare.33723265.v1 https://figshare.com/articles/dataset/Cotton_Gradient_Test/33723265 MIT