2026-10-11T16:57:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33719669 2026-09-14T07:08:10Z category_1 category_4 category_7 category_12 category_13 category_14 category_16 category_873 category_46 category_61 category_64 category_128 portal_63 item_type_3 month_year_09_2026

Identification of Gβ5 Interactome Associated with Neuronal Development in Neuro-2a Cells through miniTurbo Proximity Labeling Harrison J. McNabb (24868787) Jianhua Zhang (6347) Connor P. Jewell (24868790) Weiming Yang (433041) Lisa M. Jenkins (18549038) William F. Simonds (24868793) Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Immunology Developmental Biology Cancer Hematology two analysis methods transduce extracellular signals previously reported phenotypes multisystem developmental disorder enriched phenotypes associated cell biotin proximity protein coupled receptor rgs also localizes 2a cells coupled heterotrimeric gβ proteins gβ5 interactome associated 2a cells protein signaling gβ family well studied subcellular compartments results align plasma membrane neuronal development mass spectrometry likely important labeling assay activating proteins Gβ5 is a member of the Gβ family; however, unlike other heterotrimeric Gβ proteins, which transduce extracellular signals in response to G-protein coupled receptor (GPCR) activation, Gβ5 negatively regulates GPCR signaling by stabilizing the GTPase-activating proteins (GAP) R7 subfamily of Regulator of G-protein Signaling (R7-RGS) proteins. Mutations in Gβ5 have been implicated in a multisystem developmental disorder. While the regulation of GPCR signaling at the plasma membrane is likely important in this process, Gβ5/R7-RGS also localizes to the cytosol and nucleus. Potential protein–protein interactions of Gβ5 in these subcellular compartments are not well studied, so we sought to characterize the interactome of Gβ5 utilizing a live-cell biotin proximity-labeling assay in Neuro-2a cells coupled with mass spectrometry to identify novel protein interactions of the complex. We identified 48 potential interactors of Gβ5 with two analysis methods, most of which are previously unreported interactions important in neuronal development and function. The enriched phenotypes associated with the Gβ5 interactome in our results align with previously reported phenotypes of Gβ5 deficiencies. 2026-09-14T00:00:00Z Dataset Dataset 10.1021/acsomega.6c05064.s001 https://figshare.com/articles/dataset/Identification_of_G_5_Interactome_Associated_with_Neuronal_Development_in_Neuro-2a_Cells_through_miniTurbo_Proximity_Labeling/33719669 CC BY-NC 4.0