2026-10-14T11:03:12Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33719473 2026-09-14T06:11:47Z category_1 category_4 category_915 category_7 category_8 category_12 category_13 category_14 category_873 category_46 category_734 category_61 category_64 category_69 category_132 portal_63 item_type_3 month_year_09_2026

Toward Optimized Proteomics Analysis to Detect Bacterial and Host Proteins in Calcified Coronary Artery Plaques Kirsikka Musta (24868639) Markus J. T. Ojanen (24868642) Maria Stensland (9313721) Jonna Kankaanranta (24868645) Terho Lehtimäki (20437014) Pekka J. Karhunen (7300592) Tuula A. Nyman (221395) Vesa P. Hytönen (1337616) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Microbiology Cell Biology Genetics Molecular Biology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Developmental Biology Cancer Inorganic Chemistry Infectious Diseases significantly increased morbidity extraction methods showed coronary heart disease molecular microbial techniques coronary artery plaques based extraction method 24 bacterial proteins reliable method molecular pathology atherosclerotic plaques detect bacterial bacterial proteins scale analysis proteome level poorly understood oral biofilm optimal due host proteins high number forming bacteria findings provide final stage enable studying dental calculus calcified areas Coronary calcification is the final stage in coronary heart disease, associated with significantly increased morbidity and mortality. The molecular pathology of calcification and especially events triggering calcification are poorly understood. Metagenomic and molecular microbial techniques have identified the presence of oral biofilm-producing and dental calculus-forming bacteria in atherosclerotic plaques, suggesting that they may be involved in calcification. To enable studying this at the proteome level, we compared four different protein extraction methods to find a robust and reliable method for the identification and quantification of human and bacterial proteins from 10 calcified arterial plaques. We identified 3 913 human proteins and 24 bacterial proteins. These findings provide further support for the presence of bacterial proteins in calcified areas of coronary artery plaques, with Fusobacteria being the most abundant bacterial protein source. Comparisons of the extraction methods showed that the urea-based extraction method was most optimal due to its simplicity and reproducible identification of a high number of proteins, which makes it suitable for further large-scale analysis of coronary artery plaque samples. 2026-09-14T00:00:00Z Dataset Dataset 10.1021/acs.jproteome.6c00422.s002 https://figshare.com/articles/dataset/Toward_Optimized_Proteomics_Analysis_to_Detect_Bacterial_and_Host_Proteins_in_Calcified_Coronary_Artery_Plaques/33719473 CC BY-NC 4.0