2026-10-14T08:17:40Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33718993 2026-09-14T05:40:31Z category_8 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_An unfolded protein response-inducible protein (ULI1) mediates aromatic alcohols biosynthesis through reinforcing the Ehrlich pathway in Saccharomyces cerevisiae.zip Xiaowei Gong (528870) Yong Shen (284972) Shanzhai Shang (4780794) Jianbo Zhan (7299221) Guanghui Ma (1428790) Huajun Luo (10661990) Shiwei Li (1401982) Chunxia Song (364885) Donglai Zhu (10589246) Yiyong Luo (6170300) Microbiology 2-phenylethanol gene editing proteomics Saccharomyces cerevisiae transcriptomics tryptophol unfolded protein response <p>Aromatic alcohols such as 2-phenylethanol (2-PE) and tryptophol (TOL) possess diverse biological functions. Here, an unfolded protein response-inducible gene ULI1 and an aldehyde dehydrogenase gene ALD3 in Saccharomyces cerevisiae were disrupted using the CRISPR-Cas9 system. Fermentation assays revealed that both the ULI1 frameshift (Kmuli1) and knockout (KΔuli1) mutants exhibited significantly reduced 2-PE and TOL yields relative to the wild-type strain KMLY1-6, retaining 46.32 and 64.54% for 2-PE, and 34.61 and 77.73% for TOL, respectively. In contrast, ALD3 knockout moderately elevated 2-PE and TOL production by 4.98 and 20.57%, respectively. These results indicate that both ULI1 and ALD3 mediate the biosynthesis of 2-PE and TOL in S. cerevisiae. To further confirm the role of ULI1, three chromosomal integration expression (CIE) strains (KXuli1, KXIIuli1, KΔald3uli1) and one plasmid-based expression (PBE) strain (BY4741/pY26-uli1) were constructed. Compared with the control KMLY1-6, the CIE strains increased 2-PE and TOL production by 10.13–12.91% and 25.76–29.28%, respectively. Meanwhile, the PBE strain produced significantly higher levels of 2-PE and TOL than the empty vector control (BY4741/pY26), with maximum increases of 38.80% and 30.86% detected at 60 h of cultivation. To further elucidate the molecular mechanism of ULI1 in 2-PE and TOL biosynthesis, comparative transcriptomic analysis for Kmuli1 vs. KMLY1-6, together with transcriptomic and proteomic analyses for BY4741/pY26-uli1 vs. BY4741/pY26, was performed. Multi-omics results revealed that ULI1 reinforces the Ehrlich pathway, modulates metabolic flux, maintains mitochondrial structure and cellular protein homeostasis, and coordinates stress responses with metabolic redistribution. Collectively, ULI1 acts as a novel regulatory hub linking stress tolerance and cellular homeostasis, and facilitates aromatic alcohol biosynthesis by strengthening the Ehrlich pathway in S. cerevisiae.</p> 2026-09-14T05:40:31Z Dataset Dataset 10.3389/fmicb.2026.1934502.s001 https://figshare.com/articles/dataset/Data_Sheet_1_An_unfolded_protein_response-inducible_protein_ULI1_mediates_aromatic_alcohols_biosynthesis_through_reinforcing_the_Ehrlich_pathway_in_Saccharomyces_cerevisiae_zip/33718993 CC BY 4.0