2026-10-14T02:58:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33718774 2026-09-14T05:32:06Z category_21 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Hollow-degree-tuned Prussian blue–mesoporous silica nanoplatforms for chemo-photothermal cancer therapy with preliminary microfluidic perfusion evaluation.docx Jingjing Xi (8936492) Jingjing Liu (305549) Heyao Zhang (12321162) Mengxi Wang (231051) Chenyi Lu (17037744) Tinghan Wang (24868132) Xijun Piao (1630693) Ri Huang (17271975) Keyun Ren (24868135) Xiao Mei (12884465) Biotechnology chemo-photothermal therapy drug delivery hollow-degree engineering mesoporous silica microfluidic evaluation prussian blue Background<p>Balancing photothermal performance and drug-delivery capacity remains a key challenge in the structural design of multifunctional nanomedicines. Hollowing can increase internal space for drug accommodation but may simultaneously reduce the amount of NIR-absorbing Prussian blue (PB), creating an inherent trade-off between drug-delivery capacity and photothermal performance.</p>Methods<p>The hollow state of hollow mesoporous Prussian blue (HMPB) nanocubes was regulated by controlled HCl etching, followed by a common mesoporous silica (mSiO₂) coating, to generate low-, intermediate-, and high-hollow formulations. The resulting formulations were evaluated for physicochemical properties, photothermal performance, doxorubicin (DOX) loading and release, cellular response, intracellular fluorescence, chemo-photothermal interaction, and preliminary microfluidic perfusion performance.</p>Results<p>Increasing hollowing enhanced DOX loading from 525 to 867 μg⋅mg⁻¹ and promoted drug release, but progressively reduced photothermal performance. The measured photothermal conversion efficiencies of 1-, 2-, and 3-HMPB@mSiO₂ were 23.37%, 18.62%, and 14.41%, respectively. ICP-MS showed decreasing Fe contents with increasing hollowing, while PB-equivalent-mass-matched measurements yielded similar photothermal conversion efficiencies (16.80%, 15.80%, and 14.41%), indicating that PB abundance is a major contributor to the observed photothermal differences. DOX release was enhanced by both mild acidity and mild hyperthermia relative to pH 7.4/37 °C. After treatment-matched correction for nanoparticle-related optical interference, the blank carriers showed limited acute carrier-associated cytotoxicity in HeLa cells over 24 h, and quantitative fluorescence imaging confirmed effective intracellular DOX delivery. At an equal total formulation concentration, the intermediate-hollow 2-HMPB@mSiO₂-DOX formulation produced the strongest combined therapeutic response. Multi-dose combination-index, Bliss-independence, and Loewe-additivity analyses further supported increasingly synergistic chemo-photothermal interactions with increasing treatment effect. Preliminary syringe-pump-driven microfluidic perfusion in physiological medium preserved the principal structure-dependent trends in particle dispersion, drug leakage, and NIR heating.</p>Conclusion<p>These results demonstrate that hollow-state engineering provides a practical strategy for balancing competing photothermal and drug-delivery functions in PB-based nanoplatforms.</p> 2026-09-14T05:32:06Z Dataset Dataset 10.3389/fbioe.2026.1932857.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Hollow-degree-tuned_Prussian_blue_mesoporous_silica_nanoplatforms_for_chemo-photothermal_cancer_therapy_with_preliminary_microfluidic_perfusion_evaluation_docx/33718774 CC BY 4.0