2026-10-14T17:31:36Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33716623 2026-09-13T23:15:08Z category_24199 category_24223 category_24310 item_type_3 month_year_09_2026

<b>Chromosome-level genome assembly and annotation of the </b><b><i>Cobitis hankugensis</i></b> Ajit Kumar Patra (6705332) Ra-Yeon Myung (8733846) Yong-Jin Won (11884893) Karel Janko (6532374) Tomáš Tichopád (11824256) Seung Jae Lee (20465912) Da-Song Choi (24866298) Sequence analysis Freshwater ecology Genomics chromosome level genome assembly Cobitis hankugensis Phylogenomics <p dir="ltr">Cobitid loaches are widely distributed across Eurasia and exhibit remarkable karyotypic and reproductive diversity, with several taxa forming stable interspecific hybrid complexes. Among them, the Korean loach <i>Cobitis hankugensis</i> serves as a key evolutionary model for investigating hybridization, polyploidization, and non-Mendelian reproductive systems. Here, we present a high-continuity, chromosome-level reference genome of <i>C. hankugensis</i> generated by integrating PacBio long-reads, Illumina short reads, and Hi-C scaffolding. The final assembly spans 1.32 Gb with a scaffold N50 of 46.57 Mb (longest scaffold: 85 Mb) and a BUSCO completeness score of 98.2%. A total of 24 chromosome-scale pseudomolecules were anchored, representing the vast majority of the assembly. Structural and functional annotations identified 47,643 protein-coding genes, of which 92.6% were functionally annotated. This high-quality genome assembly provides a foundational resource for comparative genomics, karyotypic evolution, and the genetic mechanisms underlying hybridization in freshwater fishes.</p> 2026-09-13T23:15:08Z Dataset Dataset 10.6084/m9.figshare.33716623.v1 https://figshare.com/articles/dataset/_b_Chromosome-level_genome_assembly_and_annotation_of_the_b_b_i_Cobitis_hankugensis_i_b_/33716623 CC BY 4.0