2026-10-12T11:27:06Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33668839 2026-09-12T14:22:21Z category_4 category_8 category_13 category_272 category_873 category_39 category_734 category_62 category_69 portal_63 item_type_3 month_year_09_2026

A Bacterial Pathway for Homotaurine Catabolism via Sulfopropanoate Luise Pallasdies (22878316) Helen Barber (24833869) Mihwa Lee (2475868) Laura Burchill (7474193) Ho N. N. Ho (24833872) Adam W. E. Stewart (22878313) Artur Nastasovici (24833875) Hanna Sanne-Wander (24833878) Dilushini T. Rathnayake (24833881) Vinzenz Hofferek (22878322) Leila Jebeli (16028828) Taylor A. McDaniels (24833884) Malcolm J. McConville (180544) Robert A. Field (382308) Sacha Pidot (5704802) Nichollas E. Scott (159935) Yi Jin (38575) Spencer J. Williams (180548) Biochemistry Microbiology Genetics Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry redundant suyab sulfolyases expression analyses show comparatively well documented complete bacterial pathway identifies sulfopropanoate turnover complete microbial catabolism pathway connects algal bacterial pathway xenobiotic c3 tara oceans succinate dehydrogenase remained unresolved release pyruvate oxidatively deaminated organosulfonate metabolism microbial carbon metabolizing taxa metabolic enrichment lineage bacteria human gut especially roseobacter enzymes related e </ derived foods crystal structures core sulfopropanoate conserved module completing biomineralization binding protein bilophila wadsworthia associated ecosystems >- sulfoacrylate Small aminoorganosulfonates are reservoirs of carbon, nitrogen, and sulfur in marine and host-associated ecosystems. Homotaurine, a C₃-aminoorganosulfonate produced by marine algae and present in algal-derived foods, is comparatively well documented, but its complete microbial catabolism has remained unresolved. Here we identify a complete bacterial pathway for homotaurine catabolism in Caballeronia jiangsuensis strain McCoy, isolated by metabolic enrichment from soil by growth on homotaurine. Growth, proteomic, metabolomic, biochemical, structural, and heterologous-expression analyses show that homotaurine is imported and oxidatively deaminated to sulfopropanoate, then converted to sulfolactate through <i>E</i>-sulfoacrylate by SpuABCD and SpuIJ, enzymes related to succinate dehydrogenase and fumarase. Sulfolactate is cleaved by redundant SuyAB sulfolyases to release pyruvate and sulfite, which is oxidized and excreted as sulfate, completing biomineralization. Crystal structures of the HtaA homotaurine-binding protein and SuyAB2 sulfolyase reveal ligand recognition and provide a structural framework for sulfolactate cleavage. Hidden Markov model searches and Tara Oceans and human gut metagenomes show that the core sulfopropanoate-to-sulfolactate module is widespread in marine Proteobacteria, especially Roseobacter-lineage bacteria, and occurs more narrowly in human gut-associated sulfur-metabolizing taxa such as Bilophila wadsworthia. This pathway connects algal, dietary, and xenobiotic C3-aminoorganosulfonates to microbial carbon, nitrogen, and sulfur cycling, and identifies sulfopropanoate turnover as a conserved module in organosulfonate metabolism. 2026-09-12T00:00:00Z Dataset Dataset 10.1021/jacs.6c10662.s002 https://figshare.com/articles/dataset/A_Bacterial_Pathway_for_Homotaurine_Catabolism_via_Sulfopropanoate/33668839 CC BY-NC 4.0