2026-10-07T09:28:42Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33617434 2026-10-05T11:26:34Z category_24187 item_type_3 month_year_10_2026

Chromosome-level genome assembly and karyotype validation of the tea tussock moth Euproctis pseudoconspersa yesong ren (17934290) Biological network analysis genomic alterations Telomerase reactivation <p dir="ltr">The tea tussock moth, Euproctis pseudoconspersa, is an important defoliating pest of tea plants and various economically important crops. However, nuclear genomic resources suitable for investigating chromosome evolution, population genetics, and host adaptation in this species remain limited. In this study, we integrated PacBio HiFi, Hi-C, and Illumina RNA-sequencing data to generate a chromosome-level reference genome for E. pseudoconspersaand obtained independent cytogenetic evidence through the preparation of mitotic metaphase chromosomes. The final assembly spanned 356.85 Mb, with contig and scaffold N50 values of 10.22 and 16.80 Mb, respectively. In total, 99.9% of the assembled sequence was anchored to 22 chromosome-scale pseudomolecules. Karyotype analysis revealed a diploid chromosome number of 2n = 44, consistent with the 22 haploid chromosome-scale pseudomolecules supported by the Hi-C data. Genome completeness was estimated at 99.0% using BUSCO and 99.12% using compleasm, while the Merqury quality values exceeded 67.42. A total of 14,216 protein-coding genes were predicted. The raw sequencing data, chromosome-level genome assembly, karyotype images, gene annotations, and genome quality-assessment files generated in this study have been made publicly available. These resources provide a robust and reusable foundation for comparative genomics, chromosome evolution, host adaptation, and pest-management research in E. pseudoconspersaand related lepidopteran insects.</p> 2026-10-05T11:26:34Z Dataset Dataset 10.6084/m9.figshare.33617434.v1 https://figshare.com/articles/dataset/Chromosome-level_genome_assembly_and_karyotype_validation_of_the_tea_tussock_moth_Euproctis_pseudoconspersa/33617434 CC BY 4.0