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        <identifier>oai:figshare.com:article/33427390</identifier>
        <datestamp>2026-10-01T14:59:20Z</datestamp>
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          <dc:title>Conservation genomics of the Red Roman, Chrysoblephus laticeps, across Marine Protected Areas (MPAs) and exploited regions along the South African coastline</dc:title>
          <dc:creator>Mannda Ndou (24695458)</dc:creator>
          <dc:subject>Genomics</dc:subject>
          <dc:subject>Flye</dc:subject>
          <dc:subject>Interproscan annotation</dc:subject>
          <dc:subject>Oxford nanopore sequencing (ONT)</dc:subject>
          <dc:subject>Polypolish</dc:subject>
          <dc:subject>Variant call format (VCF)</dc:subject>
          <dc:subject>Reference genome B</dc:subject>
          <dc:subject>Blast annotations</dc:subject>
          <dc:subject>Scaffold level</dc:subject>
          <dc:subject>Eggnog function composition</dc:subject>
          <dc:subject>Chrysoblephus laticeps</dc:subject>
          <dc:subject>Marine protected areas (MPAs)</dc:subject>
          <dc:subject>Conservation genomics</dc:subject>
          <dc:subject>Red Roman</dc:subject>
          <dc:subject>South African coastline</dc:subject>
          <dc:subject>SDG 13 Climate action</dc:subject>
          <dc:subject>SDG 14 Life below water</dc:subject>
          <dc:description>&lt;p dir="ltr"&gt;This study was developed to investigate how MPAs are contributing to shaping the evolutionary history of Red Roman across their core distribution range. This research utilises VCF files generated with BCFtools from whole-genome sequences of the Red Roman seabream, obtained through DNBSEQ sequencing technology. The goal was to conduct genomic analyses to support the conservation of the species across its distribution range, including Marine Protected Areas and exploited regions along the South African coastline. The reference genome was assembled using Flye Assembler and subsequently polished with Polca and Polypolish. Structural annotation was performed using Braker3, while functional annotation was performed using a combination of BlastN, InterProScan, and EggNOG mapper. The ten sample VCF files correspond to ten Red Roman samples mapped to Chrysoblephus laticeps, Pagrus major, Sparus aurata, and Acanthopagrus latus for the comparison of genetic metric estimates.&lt;/p&gt;</dc:description>
          <dc:date>2026-10-01T14:59:20Z</dc:date>
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          <dc:identifier>10.25403/UPresearchdata.33427390.v1</dc:identifier>
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          <dc:rights>CC BY 4.0</dc:rights>
          <dc:rights>Open Access after 2028-09-04</dc:rights>
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