2026-10-12T05:14:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33387850 2026-09-23T03:30:03Z category_24367 portal_886 item_type_3 month_year_09_2026

Supporting data for "Population immunity to antigenically distinct N1 neuraminidases derived from HxN1 human, avian and swine influenza viruses" Jiaying Li (19238446) Virology Influenza neuraminidase population immunity <p dir="ltr">This project characterizes the antigenic relationships among N1 neuraminidases (NA) derived from human, swine, and avian HxN1 viruses, while simultaneously evaluating cross-reactive population immunity in both adult and paediatric cohorts. To map the N1 antigenic landscape, we generated 12 recombinant H6N1 viruses to enable a focused assessment of NA antigenic diversity. The accompanying datasets are organized sequentially to align with the thesis and include the following five files:</p><ol><li>A phylogenetic tree illustrating the genetic relationships of 466 N1 NA sequences, annotated by host origin.</li><li>Normalization calculations used to standardize and validate the NA activity of the recombinant viruses.</li><li>Neuraminidase inhibition (NAI) titres measured via Enzyme-Linked Lectin Assay (ELLA) using post-infection ferret antisera, defining the antigenic relationships among the 12 selected N1 viruses.</li><li>An antigenic cartography map generated from the ferret NAI data, alongside correlation analyses comparing antigenic distances to (i) phylogenetic tip-to-tip distances, (ii) overall amino acid sequence dissimilarity, and (iii) the number of substitutions at defined antigenic sites.</li><li>Population-level NAI titres measured via ELLA in human cohorts: an adult cohort (n=120, ages 18–65, sera collected 2023–2024) tested against 8 viruses, and a paediatric cohort (n=20, ages 4–6, sera collected 2021) tested against 4 viruses, to assess cross-reactive immunity.</li></ol><p></p> 2026-09-23T03:30:03Z Dataset Dataset 10.25442/hku.33387850.v1 https://figshare.com/articles/dataset/Supporting_data_for_Population_immunity_to_antigenically_distinct_N1_neuraminidases_derived_from_HxN1_human_avian_and_swine_influenza_viruses_/33387850 CC BY-NC 4.0 Open Access after 2027-10-31