2026-10-06T11:33:58Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33349743 2026-09-17T13:58:23Z category_24148 category_24160 portal_918 item_type_3 month_year_09_2026

Increased mechanical load remodels the glomerular matrix and accelerates Alport kidney disease: processed data and analysis code Franziska Lausecker (17122256) Rafaella Konstantinou (16024997) Shanshan anna Li (24576927) Michael Joseph Randles (4525006) Maryline Fresquet (21018995) Emily Williams (11461711) Bernard Davenport (10667916) Penny L. Hubbard Cristinacce (20610354) Ben R. Dickie (15274264) Craig Lawless (10806707) Christoph Ballestrem (16445882) Rachel Lennon (8928569) Cellular interactions (incl. adhesion, matrix, cell wall) Proteomics and intermolecular interactions (excl. medical proteomics) glomerulus glomerular basement membrane (GBM) extracellular matrix matrisome collagen IV Col4a5 Alport syndrome podocyte unilateral nephrectomy nephron loss hyperfiltration compensatory hypertrophy mechanotransduction CCN2 CTGF YAP/TAZ mass spectrometry proteomics label-free quantification electron microscopy serial block-face SEM DCE-MRI mouse model <p dir="ltr">Processed data and analysis code supporting a study of how the glomerulus responds to increased mechanical load, and why that response becomes damaging when the glomerular basement membrane is structurally abnormal. </p><p dir="ltr">Unilateral nephrectomy was performed in wild-type and Col4a5-deficient (Alport) mice, and the remaining kidney was followed across scales: dynamic contrast-enhanced MRI for haemodynamic and structural adaptation; transmission and serial block-face electron microscopy for GBM thickness, foot-process/GBM ratio and podocyte protrusion frequency; mass-spectrometry proteomics of matrix-enriched and cell-enriched glomerular fractions; and functional assays in primary wild-type and Alport podocytes.</p><p dir="ltr"><br></p> 2026-09-17T13:58:23Z Dataset Dataset 10.48420/33349743.v2 https://figshare.com/articles/dataset/Increased_mechanical_load_remodels_the_glomerular_matrix_and_accelerates_Alport_kidney_disease_processed_data_and_analysis_code/33349743 CC BY 4.0