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        <identifier>oai:figshare.com:article/33346458</identifier>
        <datestamp>2026-09-14T04:20:58Z</datestamp>
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          <dc:title>Deep amplicon sequencing data in off-target detection and sgRNA editing efficiency in wheat protoplasts</dc:title>
          <dc:creator>shenglong bai (19715848)</dc:creator>
          <dc:subject>Plant physiology</dc:subject>
          <dc:subject>CRISPR/Cas9</dc:subject>
          <dc:subject>ABE base editing</dc:subject>
          <dc:subject>off-target</dc:subject>
          <dc:description>&lt;p dir="ltr"&gt;File 1, Assessment of off-target effects in the uorfTaCIPK14, uorfTaGGP1-ACG and uORFTaMADS26 mutants with indel or A-to-G editing. For each of the five on-target site, four potential off-target sites (1-4 bp mismatches) in common wheat genome were analyzed. &lt;/p&gt;&lt;p dir="ltr"&gt;File 2, Editing efficiencies of sgRNAs targeting the 5′ UTRs of TaCIPK14 and TaMADS26 in wheat protoplasts&lt;/p&gt;</dc:description>
          <dc:date>2026-09-14T00:00:00Z</dc:date>
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          <dc:identifier>10.6084/m9.figshare.33346458.v1</dc:identifier>
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