2026-10-06T11:58:14Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33324813 2026-09-22T21:30:31Z category_30563 category_30962 category_31100 category_30701 category_30698 category_31109 portal_1059 item_type_3 month_year_09_2026

Data from: Active and total rhizosphere microbiomes of plant mixtures diverge from additive expectations, depending on nitrogen and plant composition JENNIFER HARRIS (24553296) Emma Rice (24610707) Carolyn Lowry (23712045) Estelle Couradeau (24643504) Liana Burghardt (23228320) Agricultural, veterinary and food sciences Ecology Microbiology Organic and low chemical input crop production Fertilisers (incl. application) Microbial ecology plant-microbiome interactions BONCAT 16S rRNA gene amplicon data cover crops plant mixtures microbiome microbial activity <p dir="ltr">Mixing plant functional groups can boost plant production, but the underlying microbial dynamics remain unclear. In a greenhouse experiment, we tested how monocultures and mixtures of legumes, brassicas, and grasses affect plant traits and the total and active rhizosphere microbiomes under varying nitrogen levels. While shoot biomass was predictable from monocultures, legume-containing mixtures yielded greater-than-expected root biomass and N-fixation. BONCAT-FACS, coupled with 16S rRNA gene amplicon sequencing, revealed that each plant species supports distinct active and total microbiomes, and nitrogen addition increased divergence among plant species and their mixtures in the total microbiome. In plant mixtures, microbiomes were often additive combinations of those associated with the individual species. However, legume–grass combinations frequently induced non-additive shifts in microbial composition, microbial activity, and the abundance of <i>Rhizobium</i> sp. The concurrence of non-additive plant benefits and non-additive shifts in microbial composition and activity in legume-grass mixtures suggests that emergent plant-microbe interactions may underlie synergistic belowground responses. </p><p dir="ltr">This database included multiple data types, including plant biomass, nitrogen isotope abundance, weed seed germination, BONCAT microbial activity, and processed 16S rRNA gene amplicon sequencing data. Readme files are present for each data type.</p> 2026-09-22T21:30:31Z Dataset Dataset 10.15482/USDA.ADC/33324813.v1 https://figshare.com/articles/dataset/Data_from_Active_and_total_rhizosphere_microbiomes_of_plant_mixtures_diverge_from_additive_expectations_depending_on_nitrogen_and_plant_composition/33324813 CC0