2026-10-11T14:40:23Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33324522 2026-09-27T15:38:48Z category_24172 category_26917 category_24370 item_type_3 month_year_09_2026

A CRISPR-SHERLOCK-based biosensor detecting mcyE and mcyB microcystin synthase gene RNA transcripts as an early sensing system for microcystin production Aneesha Aryan (24643218) Ren Gao (11201118) Synthetic biology Environmental biotechnology diagnostics (incl. biosensors) Microbiology not elsewhere classified CRISPR-cas13a SHERLOCK microcystin harmful algal blooms mycE mycB RNA biosensor cyanobacteria <p dir="ltr">Raw plate-reader fluorescence data from three independent CRISPR-Cas13a (SHERLOCK) experiments testing crRNA performance against microcystin synthetase gene transcripts. Experiments were run on 3 August, 17 August and 18 August 2026 by the BOSLab iGEM 2025 team.<br><br>Seven crRNAs were tested: bot1, bot2 and bot3 (targeting mcyB), eot1, eot2 and eot3 (targeting mcyE), and 16sg (16S rRNA control guide). Each crRNA was run in triplicate under three conditions: normal (target RNA present), no guide, and mismatch. Control wells included no-Cas, probe-only and RNase conditions. Fluorescence was read at 5-minute intervals through a 60-minute reaction endpoint. The platemap image shows the well layout used in all three runs.<br><br>Files: <br>- 8_03_plate_reader_results.xlsx: raw plate-reader output, experiment 1 <br>- 8_17_plate_reading_results.xlsx: raw plate-reader output, experiment 2 <br>- 8_18_plate_reading_results.xlsx: raw plate-reader output, experiment 3 <br>- platemap.png: plate layout and well assignments</p> 2026-09-26T00:00:00Z Dataset Dataset 10.6084/m9.figshare.33324522.v1 https://figshare.com/articles/dataset/A_CRISPR-SHERLOCK-based_biosensor_detecting_mcyE_and_mcyB_microcystin_synthase_gene_RNA_transcripts_as_an_early_sensing_system_for_microcystin_production/33324522 CC BY 4.0