2026-10-09T18:55:54Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33314883 2026-09-22T06:43:51Z category_24892 category_24139 category_24289 portal_886 item_type_3 month_year_09_2026

<b>Supporting Data for </b><b><i>"METTL3-dependent Centromeric RNA-m6A </i></b> Xiangyu Ouyang (13008891) Other biomedical and clinical sciences not elsewhere classified Cell development, proliferation and death Cell and nuclear division mitosis METTL3 embryonic stem cell genomic integrity pre-implantation embryonic development RNA m6A modification CENPA centromere alpha satellite <p dir="ltr">METTL3-dependent RNA m6A regulates developmental competence and mitotic integrity in early human stem-cell models. Across hEPSCs, hESCs, hTSCs, blastoids, and donated embryos, METTL3 perturbation impaired stem-cell fitness, pluripotency exit, trophoblast competence and developmental progression. In hTSCs, METTL3 supported cytotrophoblast-like identity through epigenetic regulation involving EP300, JUND and EZH2. Mitotic abnormalities, chromosome missegregation and altered DNA content were particularly prominent in early stem-cell states. METTL3 loss also disrupted CENPA maintenance and centromeric alpha-satellite RNA regulation, including ALR m6A, chromatin state, and CENPA/YTHDC1-associated enrichment. These findings identify a developmental-stage-associated METTL3 dependency with implications for genome-quality control and developmental safety.</p> 2026-09-22T06:43:51Z Dataset Dataset 10.25442/hku.33314883.v1 https://figshare.com/articles/dataset/_b_Supporting_Data_for_b_b_i_METTL3-dependent_Centromeric_RNA-m6A_i_b_/33314883 CC BY-NC 4.0 Open Access after 2028-08-22